WO2004024125A1 - Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same - Google Patents
Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same Download PDFInfo
- Publication number
- WO2004024125A1 WO2004024125A1 PCT/US2003/029272 US0329272W WO2004024125A1 WO 2004024125 A1 WO2004024125 A1 WO 2004024125A1 US 0329272 W US0329272 W US 0329272W WO 2004024125 A1 WO2004024125 A1 WO 2004024125A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- pharmaceutical composition
- polypeptide
- composition
- rhil
- subject
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 126
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 40
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 37
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims description 54
- 230000003111 delayed effect Effects 0.000 title claims description 12
- 238000009472 formulation Methods 0.000 title description 72
- 239000003814 drug Substances 0.000 title description 15
- 229940124597 therapeutic agent Drugs 0.000 title description 3
- 102000003815 Interleukin-11 Human genes 0.000 claims abstract description 65
- 108090000177 Interleukin-11 Proteins 0.000 claims abstract description 65
- 229940074383 interleukin-11 Drugs 0.000 claims abstract description 48
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 35
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 34
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 34
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical group OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 33
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 30
- 239000010410 layer Substances 0.000 claims description 21
- 238000007789 sealing Methods 0.000 claims description 18
- 229940117841 methacrylic acid copolymer Drugs 0.000 claims description 17
- 229920003145 methacrylic acid copolymer Polymers 0.000 claims description 17
- 229930182817 methionine Natural products 0.000 claims description 17
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 16
- 239000004471 Glycine Substances 0.000 claims description 15
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 14
- 229920000053 polysorbate 80 Polymers 0.000 claims description 14
- 230000000975 bioactive effect Effects 0.000 claims description 13
- 239000002775 capsule Substances 0.000 claims description 13
- 239000002702 enteric coating Substances 0.000 claims description 12
- 238000009505 enteric coating Methods 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 11
- 235000001014 amino acid Nutrition 0.000 claims description 11
- 150000001720 carbohydrates Chemical class 0.000 claims description 11
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 11
- 229940068968 polysorbate 80 Drugs 0.000 claims description 11
- 239000005720 sucrose Substances 0.000 claims description 11
- 239000000454 talc Substances 0.000 claims description 11
- 229910052623 talc Inorganic materials 0.000 claims description 11
- 239000004014 plasticizer Substances 0.000 claims description 10
- 239000011230 binding agent Substances 0.000 claims description 9
- -1 cysteine amino acid Chemical class 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- DOOTYTYQINUNNV-UHFFFAOYSA-N Triethyl citrate Chemical group CCOC(=O)CC(O)(C(=O)OCC)CC(=O)OCC DOOTYTYQINUNNV-UHFFFAOYSA-N 0.000 claims description 8
- 239000001069 triethyl citrate Substances 0.000 claims description 8
- VMYFZRTXGLUXMZ-UHFFFAOYSA-N triethyl citrate Natural products CCOC(=O)C(O)(C(=O)OCC)C(=O)OCC VMYFZRTXGLUXMZ-UHFFFAOYSA-N 0.000 claims description 8
- 235000013769 triethyl citrate Nutrition 0.000 claims description 8
- 150000001413 amino acids Chemical group 0.000 claims description 7
- 206010009900 Colitis ulcerative Diseases 0.000 claims description 6
- 208000011231 Crohn disease Diseases 0.000 claims description 6
- 230000004988 N-glycosylation Effects 0.000 claims description 6
- 201000006704 Ulcerative Colitis Diseases 0.000 claims description 6
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 6
- 235000018417 cysteine Nutrition 0.000 claims description 6
- 208000022559 Inflammatory bowel disease Diseases 0.000 claims description 5
- 101001010568 Homo sapiens Interleukin-11 Proteins 0.000 claims description 4
- 230000008512 biological response Effects 0.000 claims description 4
- 102000049885 human IL11 Human genes 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 3
- 210000000813 small intestine Anatomy 0.000 claims description 3
- 125000000185 sucrose group Chemical group 0.000 claims description 3
- 229920006318 anionic polymer Polymers 0.000 claims description 2
- 230000003381 solubilizing effect Effects 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 43
- 239000006186 oral dosage form Substances 0.000 claims 4
- 208000027866 inflammatory disease Diseases 0.000 claims 2
- 239000007903 gelatin capsule Substances 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 241000700159 Rattus Species 0.000 description 63
- 239000003826 tablet Substances 0.000 description 44
- 102000012153 HLA-B27 Antigen Human genes 0.000 description 40
- 108010061486 HLA-B27 Antigen Proteins 0.000 description 40
- 230000000694 effects Effects 0.000 description 40
- 229940068196 placebo Drugs 0.000 description 34
- 239000000902 placebo Substances 0.000 description 34
- 238000004090 dissolution Methods 0.000 description 32
- 210000003205 muscle Anatomy 0.000 description 27
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 26
- 241000894007 species Species 0.000 description 24
- 239000008188 pellet Substances 0.000 description 23
- 238000011282 treatment Methods 0.000 description 20
- 239000000243 solution Substances 0.000 description 18
- 230000008602 contraction Effects 0.000 description 17
- 239000000546 pharmaceutical excipient Substances 0.000 description 17
- 102000004127 Cytokines Human genes 0.000 description 16
- 108090000695 Cytokines Proteins 0.000 description 16
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 16
- 230000000968 intestinal effect Effects 0.000 description 13
- 210000001630 jejunum Anatomy 0.000 description 13
- 235000019359 magnesium stearate Nutrition 0.000 description 13
- 229920000642 polymer Polymers 0.000 description 13
- 230000004044 response Effects 0.000 description 13
- 102000003896 Myeloperoxidases Human genes 0.000 description 12
- 108090000235 Myeloperoxidases Proteins 0.000 description 12
- 239000003963 antioxidant agent Substances 0.000 description 12
- 235000006708 antioxidants Nutrition 0.000 description 12
- 229940079593 drug Drugs 0.000 description 12
- 206010061218 Inflammation Diseases 0.000 description 11
- AIXAANGOTKPUOY-UHFFFAOYSA-N carbachol Chemical compound [Cl-].C[N+](C)(C)CCOC(N)=O AIXAANGOTKPUOY-UHFFFAOYSA-N 0.000 description 11
- 229960004484 carbachol Drugs 0.000 description 11
- 230000004054 inflammatory process Effects 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 210000001072 colon Anatomy 0.000 description 10
- 230000000112 colonic effect Effects 0.000 description 10
- 238000009477 fluid bed granulation Methods 0.000 description 10
- 235000018102 proteins Nutrition 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 206010012735 Diarrhoea Diseases 0.000 description 9
- 239000008363 phosphate buffer Substances 0.000 description 9
- 230000003078 antioxidant effect Effects 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 238000007907 direct compression Methods 0.000 description 8
- 239000012738 dissolution medium Substances 0.000 description 8
- 230000003628 erosive effect Effects 0.000 description 8
- 238000003860 storage Methods 0.000 description 8
- 238000013268 sustained release Methods 0.000 description 8
- 239000012730 sustained-release form Substances 0.000 description 8
- 239000002202 Polyethylene glycol Substances 0.000 description 7
- 238000000576 coating method Methods 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 230000000284 resting effect Effects 0.000 description 7
- 229910000162 sodium phosphate Inorganic materials 0.000 description 7
- 235000000346 sugar Nutrition 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 229940024606 amino acid Drugs 0.000 description 6
- 235000014633 carbohydrates Nutrition 0.000 description 6
- 239000011248 coating agent Substances 0.000 description 6
- 230000009989 contractile response Effects 0.000 description 6
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 6
- 238000005469 granulation Methods 0.000 description 6
- 230000003179 granulation Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 230000004118 muscle contraction Effects 0.000 description 6
- 229920001223 polyethylene glycol Polymers 0.000 description 6
- 239000008213 purified water Substances 0.000 description 6
- 239000001488 sodium phosphate Substances 0.000 description 6
- 235000011008 sodium phosphates Nutrition 0.000 description 6
- 239000007916 tablet composition Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 5
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 229910000397 disodium phosphate Inorganic materials 0.000 description 5
- 239000002662 enteric coated tablet Substances 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000008108 microcrystalline cellulose Substances 0.000 description 5
- 229940016286 microcrystalline cellulose Drugs 0.000 description 5
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 239000007939 sustained release tablet Substances 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 4
- 238000003287 bathing Methods 0.000 description 4
- 238000004166 bioassay Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000007884 disintegrant Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 230000013595 glycosylation Effects 0.000 description 4
- 238000006206 glycosylation reaction Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 230000003902 lesion Effects 0.000 description 4
- 239000000314 lubricant Substances 0.000 description 4
- KBOPZPXVLCULAV-UHFFFAOYSA-N mesalamine Chemical compound NC1=CC=C(O)C(C(O)=O)=C1 KBOPZPXVLCULAV-UHFFFAOYSA-N 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 235000021317 phosphate Nutrition 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 230000002269 spontaneous effect Effects 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 230000009261 transgenic effect Effects 0.000 description 4
- 238000005550 wet granulation Methods 0.000 description 4
- 239000004254 Ammonium phosphate Substances 0.000 description 3
- 229930003347 Atropine Natural products 0.000 description 3
- 229920003084 Avicel® PH-102 Polymers 0.000 description 3
- 229920003134 Eudragit® polymer Polymers 0.000 description 3
- RKUNBYITZUJHSG-UHFFFAOYSA-N Hyosciamin-hydrochlorid Natural products CN1C(C2)CCC1CC2OC(=O)C(CO)C1=CC=CC=C1 RKUNBYITZUJHSG-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 3
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 3
- 235000019289 ammonium phosphates Nutrition 0.000 description 3
- RKUNBYITZUJHSG-SPUOUPEWSA-N atropine Chemical compound O([C@H]1C[C@H]2CC[C@@H](C1)N2C)C(=O)C(CO)C1=CC=CC=C1 RKUNBYITZUJHSG-SPUOUPEWSA-N 0.000 description 3
- 229960000396 atropine Drugs 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 230000001713 cholinergic effect Effects 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 229960000913 crospovidone Drugs 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 3
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 3
- 235000019800 disodium phosphate Nutrition 0.000 description 3
- 238000007922 dissolution test Methods 0.000 description 3
- 239000002552 dosage form Substances 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- ACGDKVXYNVEAGU-UHFFFAOYSA-N guanethidine Chemical compound NC(N)=NCCN1CCCCCCC1 ACGDKVXYNVEAGU-UHFFFAOYSA-N 0.000 description 3
- 229960003602 guanethidine Drugs 0.000 description 3
- 239000012729 immediate-release (IR) formulation Substances 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 description 3
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 3
- 238000010254 subcutaneous injection Methods 0.000 description 3
- 239000007929 subcutaneous injection Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 206010016654 Fibrosis Diseases 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 2
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 102000002265 Human Growth Hormone Human genes 0.000 description 2
- 108010000521 Human Growth Hormone Proteins 0.000 description 2
- 239000000854 Human Growth Hormone Substances 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 2
- 229920003091 Methocel™ Polymers 0.000 description 2
- 229920003094 Methocel™ K4M Polymers 0.000 description 2
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003466 anti-cipated effect Effects 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 208000019902 chronic diarrheal disease Diseases 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- DOIRQSBPFJWKBE-UHFFFAOYSA-N dibutyl phthalate Chemical compound CCCCOC(=O)C1=CC=CC=C1C(=O)OCCCC DOIRQSBPFJWKBE-UHFFFAOYSA-N 0.000 description 2
- 150000002016 disaccharides Chemical class 0.000 description 2
- 231100000673 dose–response relationship Toxicity 0.000 description 2
- 230000004761 fibrosis Effects 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 238000009478 high shear granulation Methods 0.000 description 2
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 2
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000008176 lyophilized powder Substances 0.000 description 2
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000013563 matrix tablet Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 229960004963 mesalazine Drugs 0.000 description 2
- 235000006109 methionine Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 229940124531 pharmaceutical excipient Drugs 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 229940069328 povidone Drugs 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 238000012430 stability testing Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- CFMYXEVWODSLAX-QOZOJKKESA-N tetrodotoxin Chemical compound O([C@@]([C@H]1O)(O)O[C@H]2[C@@]3(O)CO)[C@H]3[C@@H](O)[C@]11[C@H]2[C@@H](O)N=C(N)N1 CFMYXEVWODSLAX-QOZOJKKESA-N 0.000 description 2
- 229950010357 tetrodotoxin Drugs 0.000 description 2
- CFMYXEVWODSLAX-UHFFFAOYSA-N tetrodotoxin Natural products C12C(O)NC(=N)NC2(C2O)C(O)C3C(CO)(O)C1OC2(O)O3 CFMYXEVWODSLAX-UHFFFAOYSA-N 0.000 description 2
- URAYPUMNDPQOKB-UHFFFAOYSA-N triacetin Chemical compound CC(=O)OCC(OC(C)=O)COC(C)=O URAYPUMNDPQOKB-UHFFFAOYSA-N 0.000 description 2
- 230000036269 ulceration Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 229920000623 Cellulose acetate phthalate Polymers 0.000 description 1
- 229930186147 Cephalosporin Natural products 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical group OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 108700034637 EC 3.2.-.- Proteins 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 229920003138 Eudragit® L 30 D-55 Polymers 0.000 description 1
- 229920003136 Eudragit® L polymer Polymers 0.000 description 1
- 229920003151 Eudragit® RL polymer Polymers 0.000 description 1
- 229920003152 Eudragit® RS polymer Polymers 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 102220570135 Histone PARylation factor 1_L30D_mutation Human genes 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- UETNIIAIRMUTSM-UHFFFAOYSA-N Jacareubin Natural products CC1(C)OC2=CC3Oc4c(O)c(O)ccc4C(=O)C3C(=C2C=C1)O UETNIIAIRMUTSM-UHFFFAOYSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- GGLZPLKKBSSKCX-YFKPBYRVSA-N L-ethionine Chemical compound CCSCC[C@H](N)C(O)=O GGLZPLKKBSSKCX-YFKPBYRVSA-N 0.000 description 1
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 1
- 206010025476 Malabsorption Diseases 0.000 description 1
- 208000004155 Malabsorption Syndromes Diseases 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 229920003095 Methocel™ K15M Polymers 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000004989 O-glycosylation Effects 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229920001800 Shellac Polymers 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000002216 antistatic agent Substances 0.000 description 1
- 229940072224 asacol Drugs 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000008364 bulk solution Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 229940105329 carboxymethylcellulose Drugs 0.000 description 1
- 229940081734 cellulose acetate phthalate Drugs 0.000 description 1
- 229940124587 cephalosporin Drugs 0.000 description 1
- 150000001780 cephalosporins Chemical class 0.000 description 1
- 230000000973 chemotherapeutic effect Effects 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 206010009887 colitis Diseases 0.000 description 1
- 210000004953 colonic tissue Anatomy 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000007891 compressed tablet Substances 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 230000037020 contractile activity Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 229960001334 corticosteroids Drugs 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 229940104799 dipentum Drugs 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 229940088679 drug related substance Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000005216 enteric neuron Anatomy 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- GDCRSXZBSIRSFR-UHFFFAOYSA-N ethyl prop-2-enoate;2-methylprop-2-enoic acid Chemical compound CC(=C)C(O)=O.CCOC(=O)C=C GDCRSXZBSIRSFR-UHFFFAOYSA-N 0.000 description 1
- FSXVSUSRJXIJHB-UHFFFAOYSA-M ethyl prop-2-enoate;methyl 2-methylprop-2-enoate;trimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azanium;chloride Chemical compound [Cl-].CCOC(=O)C=C.COC(=O)C(C)=C.CC(=C)C(=O)OCC[N+](C)(C)C FSXVSUSRJXIJHB-UHFFFAOYSA-M 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000001087 glyceryl triacetate Substances 0.000 description 1
- 235000013773 glyceryl triacetate Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 244000144993 groups of animals Species 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 229920001903 high density polyethylene Polymers 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000004700 high-density polyethylene Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 229940125721 immunosuppressive agent Drugs 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 229960000598 infliximab Drugs 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000003593 megakaryocyte Anatomy 0.000 description 1
- 230000028161 membrane depolarization Effects 0.000 description 1
- VAOCPAMSLUNLGC-UHFFFAOYSA-N metronidazole Chemical compound CC1=NC=C([N+]([O-])=O)N1CCO VAOCPAMSLUNLGC-UHFFFAOYSA-N 0.000 description 1
- 229960000282 metronidazole Drugs 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- DNKKLDKIFMDAPT-UHFFFAOYSA-N n,n-dimethylmethanamine;2-methylprop-2-enoic acid Chemical compound CN(C)C.CC(=C)C(O)=O.CC(=C)C(O)=O DNKKLDKIFMDAPT-UHFFFAOYSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001129 nonadrenergic effect Effects 0.000 description 1
- 230000002536 noncholinergic effect Effects 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- QQBDLJCYGRGAKP-FOCLMDBBSA-N olsalazine Chemical compound C1=C(O)C(C(=O)O)=CC(\N=N\C=2C=C(C(O)=CC=2)C(O)=O)=C1 QQBDLJCYGRGAKP-FOCLMDBBSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229940072223 pentasa Drugs 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- XNGIFLGASWRNHJ-UHFFFAOYSA-L phthalate(2-) Chemical compound [O-]C(=O)C1=CC=CC=C1C([O-])=O XNGIFLGASWRNHJ-UHFFFAOYSA-L 0.000 description 1
- 230000004983 pleiotropic effect Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229940100467 polyvinyl acetate phthalate Drugs 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 230000002586 relaxatory effect Effects 0.000 description 1
- 229940113147 shellac Drugs 0.000 description 1
- 239000004208 shellac Substances 0.000 description 1
- ZLGIYFNHBLSMPS-ATJNOEHPSA-N shellac Chemical compound OCCCCCC(O)C(O)CCCCCCCC(O)=O.C1C23[C@H](C(O)=O)CCC2[C@](C)(CO)[C@@H]1C(C(O)=O)=C[C@@H]3O ZLGIYFNHBLSMPS-ATJNOEHPSA-N 0.000 description 1
- 235000013874 shellac Nutrition 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- NCEXYHBECQHGNR-QZQOTICOSA-N sulfasalazine Chemical compound C1=C(O)C(C(=O)O)=CC(\N=N\C=2C=CC(=CC=2)S(=O)(=O)NC=2N=CC=CC=2)=C1 NCEXYHBECQHGNR-QZQOTICOSA-N 0.000 description 1
- 229960001940 sulfasalazine Drugs 0.000 description 1
- NCEXYHBECQHGNR-UHFFFAOYSA-N sulfasalazine Natural products C1=C(O)C(C(=O)O)=CC(N=NC=2C=CC(=CC=2)S(=O)(=O)NC=2N=CC=CC=2)=C1 NCEXYHBECQHGNR-UHFFFAOYSA-N 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 229960002622 triacetin Drugs 0.000 description 1
- 210000000251 trophoblastic cell Anatomy 0.000 description 1
- 210000002438 upper gastrointestinal tract Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
- 208000016261 weight loss Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/20—Interleukins [IL]
- A61K38/2073—IL-11
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
- A61K9/2886—Dragees; Coated pills or tablets, e.g. with film or compression coating having two or more different drug-free coatings; Tablets of the type inert core-drug layer-inactive layer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5073—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals having two or more different coatings optionally including drug-containing subcoatings
- A61K9/5078—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals having two or more different coatings optionally including drug-containing subcoatings with drug-free core
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
- A61K9/2806—Coating materials
- A61K9/2833—Organic macromolecular compounds
- A61K9/284—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone
- A61K9/2846—Poly(meth)acrylates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5026—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
Definitions
- the invention relates to compositions containing polypeptides, including interleukin-11, that are suitable for oral administration.
- rhIL-11 Recombinant human interleukin-11
- rhIL-11 is a non-glycosylated polypeptide of 177 amino acids. The polypeptide lacks cysteine residues and is highly basic (pi > 10.5).
- rhIL-11 is a member of a family of human growth factors that includes human growth hormone (hGH) and granulocyte colony-stimulating factor (G-CSF). rhIL-11 is used as a chemotherapeutic support agent and is administered in conjunction with other cancer treatments to increase platelet levels. rhIL-11 has also been demonstrated to have anti-inflammatory effects and to be useful in treating conditions such as Crohn's disease and ulcerative colitis.
- IL-l l is typically administered via subcutaneous injection. Formulations for subcutaneous injections must be sterile, and can be expensive relative to other routes of administration. The route is also inconvenient and uncomfortable. Subcutaneous injection has additionally been associated with complications such as local tissue damage and infection at the area of injection.
- the invention is based in part on the discovery of rhIL-11 compositions that can be delivered orally to a subject.
- the invention provides a therapeutically effective delayed release oral dosage composition that includes a bioactive polypeptide, an enteric coat (such as a methacrylic acid copolymer), and, optionally, at least one excipient.
- a bioactive polypeptide includes one or more properties selected from the group consisting of lacking an N- linked glycosylation site, having no more than one cysteine amino acid, and having a basic pi.
- the polypeptide has no cysteine residues.
- a preferred polypeptide is IL-11.
- the invention is described herein with reference to the bioactive polypeptide IL-11. However, it is understood that the features of the invention described with respect to IL-11 are also applicable to compositions and methods including other bioactive polypeptides
- the composition further includes an inert core.
- the inert core can be, e.g., a pellet, sphere or bead made up of sugar, starch, microcrystallinecellulose or any other pharmaceutically acceptable inert excipient.
- a preferred inert core is a carbohydrate, such as a monosaccharide, disaccharide, or polysaccharide, i.e., a polymer including three or more sugar molecules.
- An example of a suitable carbohydrate is sucrose. In some embodiments, the sucrose is present in the composition at a concentration of 60-75% wt/wt.
- the IL-11 layer is preferentially provided with a stabilizer such as methionine, glycine, polysorbate 80 and phosphate buffer, and/or a pharmaceutically acceptable binder, such as hydroxypropyl methylcellulose, povidone or hydroxypropylcellulose.
- a stabilizer such as methionine, glycine, polysorbate 80 and phosphate buffer
- a pharmaceutically acceptable binder such as hydroxypropyl methylcellulose, povidone or hydroxypropylcellulose.
- the composition can additionally include one or more pharmaceutical excipients.
- Such pharmaceutical excipients include, e.g., binders, disintegrants, fillers, plasticizers, lubricants, glidants, coatings and suspending/dispersing agents.
- a preferred binder is hydroxypropyl methylcellulose (HPMC).
- HPMC hydroxypropyl methylcellulose
- the HPMC is preferably present in the composition at a concentration of 3-7% wt/wt.
- a preferred glidant is talc. In some embodiments, the glidant is present in the composition at a concentration of 5-10% wt/wt.
- Plasticizers can include, e.g., triethylcitrate, polyethylene glycols, dibutyl phthalate, triacetin, dibutyl sebucate and propylene glycol.
- a preferred plasticizer is triethyl citrate.
- the triethyl citrate can be present at a concentration of 1 -2% wt/wt.
- a preferred surfactant is polysorbate 80.
- the polysorbate 80 can be present at a concentration of 0.015-0.045% wt/wt.
- the composition is provided as a multiparticulate system that includes a plurality of enteric coated, IL-11 layered pellets in a capsule dosage form.
- the enteric coated IL-11 pellets include an inert core, such as a carbohydrate sphere, a layer of IL-11 and an enteric coat.
- the enteric coat can include, e.g., a pH dependent polymer, a plasticizer, and an antisticking agent/glidant.
- Preferred polymers include, e.g., methacrylic acid copolymer, cellulose acetate phthalate, hydroxpropylmethylcellulose phthalate, polyvinyl acetate phthalate, shellac, hydroxpropylmethylcelluloseacetate succinate, carboxy-methylcellulose.
- an inert seal coat is present in the composition as a barrier between the IL-11 layer and enteric coat.
- the inert seal coat can be, e.g., hydroxypropylmethyl cellulose, povidone, hydroxypropylcellulose or another pharmaceutically acceptable binder.
- Suitable sustained release polymers include, e.g., amino methacrylate copolymers (Eudragit RL, Eudragit RS), ethylcellulose or hydroxypropyl methylcellulose.
- the methacrylic acid copolymer is a pH dependent anionic polymer solubilizing above pH 5.5.
- the methacrylic acid copolymer can be provided as a dispersion and be present in the composition at a concentration of 10-20% wt/wt.
- a preferred methacrylic acid copolymer is EUDRAGIT® L 30 D-55.
- the enteric coated tablet dosage form includes IL-11, a filler microcrystallmecellulose (Avicel PH 102), a disintegrant Explotab, a buffer sodium phosphate, an antioxidant methionine, a surfactant Tween 80, a lubricant magnesium stearate and an enteric coat .
- the sustained release tablet dosage form that includesIL-11, fillers (e.g., microcrystallinecellulose (Avicel PH 102) and sucrose), a matrix forming polymer (hydroxypropylmethylcellulose Methocel K4M Prem, Methocel KlOO LV, LH, CR, Premium), a glidant (such as Syloid), a buffer sodium phosphate, an antioxidant methionine, a surfactant (such as Tween 80), and a lubricant (such as magnesium stearate).
- fillers e.g., microcrystallinecellulose (Avicel PH 102) and sucrose
- a matrix forming polymer hydroxypropylmethylcellulose Methocel K4M Prem, Methocel KlOO LV, LH, CR, Premium
- a glidant such as Syloid
- a buffer sodium phosphate such as an antioxidant methionine
- a surfactant such as Tween 80
- a lubricant such as magnesium ste
- the composition includes glycine.
- the glycine is present in the composition at a concentration of 1-4% wt/wt.
- the composition may optionally further include an antioxidant.
- An example of a suitable antioxidant is methionine.
- the methionine is present in the composition at a concentration of 0.1-0.5% wt/wt.
- the IL-11 can be provided as a purified protein isolated from naturally occurring IL-11.
- the IL-11 polypeptide can be provided as a recombinant form of the polypeptide, e.g., recombinant human IL- 11 (rhIL- 11).
- the invention provides a therapeutically effective delayed release oral dosage multiparticulate composition including an IL-11 polypeptide, a first sealing coat, an enteric coating layer, and a second sealing coat.
- a preferred sealing coat is HPMC.
- the enteric coating layer of the composition can be, e.g., a methacrylic acid copolymer.
- a preferred methacrylic acid copolymer is soluble at a pH above 5.5, for example EUDRAGIT® L 3
- a sustained release composition that includes an IL-11 polypeptide, an enteric coat (such as a methacrylic acid copolymer), and, optionally, at least one excipient.
- the composition further includes an inert core.
- the inert core can be, e.g., a pellet, sphere or bead made up of sugar, starch, microcrystallmecellulose or any other pharmaceutically acceptable inert excipient.
- a preferred inert core is a carbohydrate, such as a monosaccharide, disaccharide, or polysaccharide, i.e., a polymer including three or more sugar molecules.
- An example of a suitable carbohydrate is sucrose. In some embodiments, the sucrose is present in the composition at a concentration of 60-75% wt/wt.
- the invention also provides a method of delivering an IL-11 polypeptide to a subject by orally administering to the subject an IL-11 polypeptide containing composition as described herein in an amount sufficient to elicit a biological response in the subject.
- the response is elicited in the small intestine of the subject.
- the subject used in the herein described method can be, e.g., a human, a non-human primate, a dog, a cat, horse, cow, pig, sheep, rabbit, rat, or mouse.
- the invention provides a method of treating or preventing inflammation in a subject by administering to the subject an oral composition that includes IL-11.
- the inflammation is associated with ulcerative colitis and Crohn's disease.
- FIG. 1 is a schematic diagram of a multi-particulate IL-11 formulation suitable for oral delivery.
- FIG. 2 is a schematic illustration of a process for making a multi-particulate IL-11 formulation suitable for oral delivery.
- the bioactive polypeptide is non-glycosylated (e.g., lacking either N-linked or O-linked glycosylation sites, or both sites), lacks a cysteine residue, and/or has a basic pi.
- the absence of glycosylation can be either because the naturally occurring polypeptide lacks sites for glycosylation or because the protein has been engineered to lack these sites.
- the polypeptide may be treated with, e.g., glycosylases to reduce or remove glycosylated residues.
- the lack of cysteine residues can occur in the naturally occurring polypeptide sequence or in a variant form of a polypeptide in which naturally occurring cysteine residues have been either deleted or replaced with non-cysteine residues.
- a preferred polypeptide for use in the formulation is interleukin 11 (IL-11).
- IL-11 is a pleiotropic cytokine that stimulates primitive lymphoheniatopoietic progenitor cells and acts in synergy with other hematopoietic growth factors to stimulate the proliferation and maturation of megakaryocytes.
- IL-11 is described in detail in International Application PCT/US90/06803, published May 30, 1991; as well as in U.S. Pat. No. 5,215,895; issued Jun. 1, 1993.
- a cloned human IL-11 was previously deposited with the ATCC, 10801 University Boulevard, Manassas, Va. 20110-2209, on Mar. 30, 1990 under ATCC No. 68284.
- IL-11 may also be produced recombinantly as a fusion protein with another protein.
- IL-11 can be produced in a variety of host cells by resort to now conventional genetic engineering techniques.
- IL-11 can be obtained from various cell lines, for example, the human lung fibroblast cell line, MRC-5 (ATCC Accession No. CCL 171) and Paul et al, the human trophoblastic cell line, TPA30-1 (ATCC Accession No. CRL 1583).
- IL-11 Described in Proc Natl Acad Sci USA 87:7512 (1990) is a cDNA encoding human IL-11 as well as the deduced amino acid sequence (amino acids 1 to 199).
- U.S. Pat. No. 5,292,646, supra describes a des-Pro form of IL-11 in which the N-terminal proline of the mature form of IL-11 (amino acids 22-199) has been removed (amino acids 23-199).
- any form of IL-11 which retains IL-11 activity, is useful according to the present invention.
- IL-11 may also be produced by known conventional chemical synthesis.
- cytokine polypeptide sequences useful in the present invention by synthetic means are known to those of skill in the art.
- the synthetically constructed cytokine polypeptide sequences by virtue of sharing primary, secondary, or tertiary structural and conformational characteristics with the natural cytokine polypeptides are anticipated to possess biological activities in common therewith.
- Such synthetically constructed cytokine polypeptide sequences or fragments thereof, which duplicate or partially duplicate the functionality thereof may also be used in the method of this invention.
- they may be employed as biologically active or immunological substitutes for the natural, purified cytokines useful in the present invention. Modifications in the protein, peptide or DNA sequences of these cytokines or active fragments thereof may also produce proteins which may be employed in the methods of this invention.
- modified cytokines can be made by one skilled in the art using known techniques. Modifications of interest in the cytokine sequences, e.g., the IL-11 sequence, may include the replacement, insertion or deletion of one or more selected amino acid residues in the coding sequences. Mutagenic techniques for such replacement, insertion or deletion are well known to one skilled in the art. (See, e.g., U.S. Pat. No. 4,518,584.)
- sequences of the cytokine polypeptides which may be useful therapeutically as described herein may involve, e.g., the insertion of one or more glycosylation sites.
- An asparagine-linked glycosylation recognition site can be inserted into the sequence by the deletion, substitution or addition of amino acids into the peptide sequence or nucleotides into the DNA sequence.
- Such changes may be made at any site of the molecule that is modified by addition of O-linked carbohydrate. Expression of such altered nucleotide or peptide sequences produces variants which may be glycosylated at those sites.
- Additional analogs and derivatives of the sequence of the selected cytokine which would be expected to retain or prolong its activity in whole or in part, and which are expected to be useful in the present method, may also be easily made by one of skill in the art.
- One such modification may be the attachment of polyethylene glycol (PEG) onto existing lysine residues in the cytokine sequence or the insertion of one or more lysine residues or other amino acid residues that can react with PEG or PEG derivatives into the sequence by conventional techniques to enable the attachment of PEG moieties.
- PEG polyethylene glycol
- Additional analogs of these selected cytokines may also be characterized by allelic variations in the DNA sequences encoding them, or induced variations in the DNA sequences encoding them. It is anticipated that all analogs disclosed in the above-referenced publications, including those characterized by DNA sequences capable of hybridizing to the disclosed cytokine sequences under stringent hybridization conditions or non-stringent conditions (Sambrook et al., Molecular Cloning. A Laboratory Manual, 2d edit., Cold Spring Harbor Laboratory, New York (1989)) will be similarly useful in this invention.
- fusion molecules prepared by fusing the sequence or a biologically active fragment of the sequence of one cytokine to another cytokine or proteinaceous therapeutic agent, e.g., IL-11 fused to IL-6 (see, e.g., methods for fusion described in PCT/US91/06186 (W092/04455), published Mar. 19, 1992).
- IL-11 a biologically active fragment of the sequence of one cytokine
- IL-6 e.g., IL-11 fused to IL-6
- combinations of the cytokines may be administered together according to the method.
- IL-11 encompasses the protein produced by the sequences presently disclosed in the art, as well as proteins characterized by the modifications described above yet which retain substantially similar activity.
- FIG. 1 A schematic diagram showing a preferred multiparticulate IL-11 formulation is shown in FIG. 1.
- a layer containing rhIL-11 On to a central sugar sphere-is disposed a layer containing rhIL-11.
- This rhIL-11 drug layer in turn is covered with a hydroxypropyl methylcellulose (HPMC) sealing coat.
- HPMC sealing coat is covered with a methacrylic acid copolymer (e.g., with Eudragit L20D-55) enteric coat, and the entire pellet is covered with a second or final HPMC sealing coat
- Oral IL-11 formulations can be prepared using any method known in the art. Examples of suitable methods include fluid bed spraying onto sucrose spheres, direct compression, and wet granulation synthetic methods. Methods of preparing compositions according to the invention are illustrated in the Examples, below.
- FIG.2 A flow diagram illustrating a preferred method for making multiparticulate IL-11 particles suitable for oral delivery is shown in FIG.2.
- the drug layer sealing coat, enteric coat, and second sealing coat are sequentially added within a fluid-bed coater. At each step temperature and mass of the formulations are preferably monitored.
- the flow diagram illustrates that sugar spheres are loaded onto a fluid-bed coater and coated with a drug layer that includes rhIL-11, sodium phosphate dibasic, sodium phosphate monobasic, glycine, polysorbate 80, methionine, hydroxypropyl methylcellulose (HPMC), and purified water to form a coat.
- a drug layer that includes rhIL-11, sodium phosphate dibasic, sodium phosphate monobasic, glycine, polysorbate 80, methionine, hydroxypropyl methylcellulose (HPMC), and purified water to form a coat.
- An enteric coat is applied containing Eudragit, talc, sodium hydroxide, triethyl citrate, and purified water.
- a seal coat of HPMC and purified water is then applied followed by talc as an anti-static agent.
- Subsequent processing can include, e.g., storage for 180 days at 2-8 degrees Centigrade.
- formulations of the invention can be delivered in any suitable form, e.g., they can be provided as capsules, sachets, tablets or suspensions.
- the formulations can be used to treat indications for which IL-11 has been demonstrated to be efficacious.
- a preferred indication is inflammatory bowel disease (IBD).
- IBD inflammatory bowel disease
- This condition is characterized by chronic intestinal inflammation that results in clinical symptoms such as diarrhea, bleeding, abdominal pain, fever, joint pain, and weight loss. These symptoms can range from mild to severe, and may gradually and subtly develop from an initial minor discomfort, or may present themselves suddenly with acute intensity.
- IBD is a prevalent cause of chronic illness in a large segment of the patient population. It can manifest itself in two different forms: Ulcerative Colitis (UC) and Crohn's Disease (CD). Although the two conditions can appear clinically very similar, UC primarily involves inflammation of the colon and rectum, as opposed to the upper GI tract. Crohn's Disease, in contrast, impacts a greater area of the upper intestinal digestive tract, and is thus more likely to trigger malabsorption, along with chronic vitamin and nutrient deficiencies.
- the oral IL-11 formulations described herein can be administered with additional agents that treat inflammatory bowel disease.
- Additional agents include, e.g., corticosteroids, immunosuppressive agents, infliximab, and mesalamine, which is a substance that helps control inflammation.
- Mesalamines include, e.g., sulfasalazine and 5-ASA agents, such as Asacol, Dipentum, or Pentasa.
- the oral IL-11 formulations can additionally be administered with antibiotics, including, for example, ampicillin, sulfonamide, cephalosporin, tetracycline, or metronidazole.
- the dosage regimen involved in a method for treating the above-described conditions will be determined by the attending physician considering various factors which modify the action of drugs, e.g. the condition, body weight, sex and diet of the patient, the severity of any infection, time of administration and other clinical factors.
- the daily regimen should be in the range of 1-30 milligrams of polypeptide.
- Example 1 Compatibility of rhIL-11 with various formulation excipients and antioxidants
- FI, F2, F4-F8 magnesium stearate
- Each tablet weighed 150 mg and contained 2.5 mg of rhIL-11 (added as lyophilized powder prepared by freeze drying the frozen concentrate in vials containing quantities equivalent to 5 mg rhIL-11 as well as sodium phosphate and glycine).
- the tablets were placed on stability at 40 C/75%RH and tested for strength and %Met oxidized species at initial, two and four weeks using a reverse phase HPLC method. In general, all the formulations studied showed an increase in % Met 58 oxidized species.
- the strength of rhIL-11 in formulation (F3) containing stearic acid dropped from initial 90.4% to 64.1% when placed on stability at 40°C/75% RH for a period of four weeks.
- antioxidants evaluated in this study were methionine, ascorbic acid and EDTA.
- the tablet formulations investigated contained 2.5 mg of rhIL-11 added as concentrate, sodium phosphate, microcrystalline cellulose, and magnesium stearate. Other ingredients are listed in Table 2. Tablets were manufactured by high shear granulation method followed by compression. Tablets were placed on stability at 40°C/75%RH and tested for % Met 58 oxidized species at initial, two and four week time points.
- Formulation (Wl) containing crospovidone but without any antioxidant produced the highest % Met 58 oxidized species.
- Formulations W2, W4, and W5 contained methionine as the antioxidant.
- the final tablet formulation was selected based on the results of excipient compatibility and antioxidant studies. Table 3 shows the formula used.
- the rhIL-11 tablets using this formula were manufactured by fluid bed granulation method. The tablets were sealed with a layer of HPMC, enteric coated with an aqueous dispersion containing Eudragit® L30D, talc and triethyl citrate and sealed again with HPMC.
- Example 2 The integrity of rhIL-11 capsules during tablet manufacturing The integrity of rhIL-11 following stresses encountered during the process of tablet manufacturing was investigated. Different compaction forces were used to evaluate the effect of tablet manufacturing stresses on the integrity of rhIL-11. These tablets weighed 150 mg, contained 2.5 mg of rhIL-11 (lyophilized powder), EXPLOTAB®, microcrystalline cellulose, NU-TAB®, syloid and magnesium stearate. Tablets were directly compressed to hardness of 2.4, 4.0, 7.5, or 12.5 KP. The protein integrity was measured by determining % recovery, % multimer, % Met oxidized species, % related and specific activity of rhIL-11 by T-10 bioassay.
- Example 3 Stability of enteric coated rhIL-11 tablets
- the dissolution test was performed in a micro-dissolution apparatus using 50 ml of glycine / phosphate dissolution medium at Paddle speed of 50 or 100 rpm.
- the coated tablets were tested for release of rhIL-11 in 0. IN HC1 for two hours followed by glycine / phosphate dissolution medium for the next 60 minutes.
- the dissolution results revealed that less than 1% rhIL-11 was released in two hours in 0.1N HC1. This suggests that 5% enteric coating is adequate in providing protection against gastric digestion.
- enteric coating was dissolved and rhIL-11 was released. As seen previously for uncoated tablets, the drug release at 50 rpm was incomplete.
- Example 4 Direct compression formulations This investigation focused on developing a sustained release tablet formulation that releases IL-11 in about 5 hours.
- Direct compression formulations were prepared as follows. Lyophilized rhIL-11 was collected, sieved through # 30 mesh screen, and transferred into a suitably sized vial containing all other excipients except magnesium stearate. Materials were blended by rotating the vial for 2-3 minutes. Magnesium stearate was added at this point and blending was continued for another 0.5 - 1 minute. Quantities of final blends equivalent to 2.5 mg rhIL-11 were weighed and compressed using a Kikusowi tableting press. Hardness was adjusted between 7 - 10 kp.
- Dissolution was conducted using the USP paddle method at 50 RPM in 150 ml of phosphate buffer pH 7.0 containing methionine, glycine, and Polysorbate 80 at 37 °C. 1 ml samples were withdrawn at predetermined time intervals and replaced with fresh medium. Analysis was conducted at ambient temperatures using a Vydac C4 column (2.1 x 150 mm, narrow bore). Flow rate was 0.5 ml per minute. Detection was performed at 214 n . A gradient system was used with 0.1 % v/v TFA as mobile phase A and 0.1 % v/v TFA in 80% acetonitrile as mobile phase B. Table 7 shows formulations of tablets prepared by direct compression.
- Example 5 Wet granulation formulations Wet granulation formulations were prepared using high sheer or fluid bed methods. rhlL-
- Dissolution was conducted using USP paddle method at 50 RPM in 150 ml of phosphate buffer pH 7.0 containing methionine, glycine, and Polysorbate 80 at 37 °C 1 ml samples were withdrawn at predetermined time intervals and replaced with fresh medium. Analysis was conducted at ambient temperatures using a Vydac C4 column (2.1 x 150 mm, narrow bore). Flow rate was 0.5 ml per minute. Detection was performed at 214 nm. A gradient system was used with 0.1 % v/v TFA as mobile phase A and 0.1% v/v TFA in 80% acetonitrile as mobile phase B.
- Sustained release formulations were prepared using granulation obtained by high sheer technique, see Table 8. A portion of drug solution was added to a blend of all excipients except polymer and magnesium stearate. The wet mass was then dried. The cycle was repeated three times to obtain targeted drug loading. The polymer was then added to the blend followed by the addition of magnesium stearate.
- the physical behavior of the tablets prepared from these formulations in the dissolution medium was found to be similar to that shown by direct compression formulations containing similar levels of HPMC. Studies with immediate release tablets prepared from high sheer granulation showed that it was difficult to obtain complete release of rhIL-11. Studies with tablets prepared from fluid bed granulation indicate that this method is the most appropriate for rhIL-11 granulation among the techniques that were investigated with respect to manufacture and release of rhIL-11.
- Table 9 shows the compositions of three sustained release tablets prepared by fluid bed granulation.
- Fluid bed granulation contain rhIL-11 mixture, Avicel PHI 02, sodium phosphate monobasic, sodium phosphate dibasic, methionine and polysorbate 80.
- sucrose which was used in the direct compression and high sheer granulation formulations was replaced with mannitol, as sucrose was found responsible for discoloring of the immediate release tablets during storage.
- Formulation 6 showed a fast initial dissociation rate in 100 mM phosphate medium.
- Formulation 6 contains Methocel K4M PREM as a sustained release polymer.
- higher viscosity grade of HPMC Metalhocel K15 M PREM
- Tablets of formulations 7 and 8 exhibited improved dissolution behavior.
- the higher rate of dissolution exhibited by formulation 8 as compared to that for formulation 7 could be due to the disintegrant properties of the extragranular microcrystalline cellulose (Avicel PHI 02), which was not present in the tablets of formulation 7.
- Matrix tablet formulations were prepared using PEO alone or in combination with HPMC. Visual evaluation of the erosion and dissolution of some of these formulations was encouraging. HPLC analysis of the dissolution samples of these formulations was difficult because of the large molecular weight of PEO.
- Prototype formulations which exhibit an optimized release profile for rhIL-11 in 50 mM phosphate medium were prepared and tested.
- Various formulations were prepared and tested. Monitoring the erosion and dissolution of these formulations indicated that using 20 - 30 % methocel KlOO LV, LH, CR Premium as a sustained release polymer might lead to obtaining formulations that exhibit an acceptable dissolution behavior.
- Table 10 shows the compositions of these formulations.
- Dissolution of rhIL-11 from formulations 9 and 10 was examined.
- the dissolution of rhIL-11 slows down significantly after two hours. Sometimes a decrease in drug concentration was noticed after two hours of dissolution.
- the incomplete release could be due to adsorption of rhIL-11 to some of the formulation excipients. This phenomenon has also been observed for immediate release tablets and beads.
- Example 8 Process for manufacturing rhIL-11 delayed release multiparticulate pellets rhIL-11 enteric-coated pellets are manufactured using a process that includes thawing and dilution of the rhIL-11 drug substance; rhIL-11 layering of the pellets; seal coating; enteric coating; final seal coating; and talc application.
- the multiparticulate pellet components are listed in Table 11.
- rhIL-11 is mixed at room temperature with dilution buffer (4 mM sodium phosphate monobasic, 6 mM sodium phosphate dibasic, 0.3 M glycine, pH 7.0) to a final concentration of 10 mg/ml.
- the diluted rhIL-11 is compounded with hydroxypropyl methylcellulose (10% solution), methionine, Polysorbate 80, and purified water to generate the drug-layering solution.
- the drug-layering solution (-40,600 g) is applied to ⁇ 20,000g of sugar spheres within a fluid-bed coater utilizing an inlet temperature range of 47-53°C, an exhaust air temperature of 30-45°C, a supply air velocity of 350-550 CFM, a spray rate of 35-85 g/min, and atomizing air at 30-40 PSI.
- a seal-coating solution ( ⁇ 2900 g) is applied to the drug-layered pellets.
- the seal-coat solution is composed of a 7.5% solution of hydroxypropyl methylcellulose in purified water (w/w).
- a fluid-bed coater is used utilizing an inlet temperature range of 47-53°C, an exhaust air temperature of 30-55°C, a supply air volume of 400-500 CFM, a spray rate of 25-45 g/min., and atomizing air at 30-40 PSI.
- the function of this seal coating is to provide an inert barrier between the rhIL-11 protein core and the acidic enteric-coating environment.
- An enteric-coating solution ( ⁇ 30,900 g) is then applied to the sealed drug-coated pellets.
- a fluid-bed coater is used utilizing an inlet temperature range of 32-38°C, an exhaust air temperature of 25-40°C, a supply air volume of 550-700 CFM, a spray rate of 45-85 g/min., and atomizing air at 25-35 PSI.
- the function of the enteric-coat layer is to provide a barrier to the acidic pH of the stomach.
- a second seal coat (-3880 g) is applied to the enteric-coated pellets.
- the seal-coat solution is composed of a 7.5 % solution of hydroxypropyl methylcellulose in purified water (w/w).
- a fluid-bed coater is used utilizing an inlet temperature range of 32-38°C, an exhaust air temperature of 25-40°C, a supply air volume of 550-700 CFM, a spray rate of 25-45 g/min., and atomizing air at 25-35 PSI.
- the function of the final seal-coat layer is to eliminate potential pellet-to-pellet sticking of the enteric-coat layer.
- the seal-coat layer is soluble in acid and is removed by the first step in the dissolution test.
- enteric coated multiparticulate pellets prepared by fluid bed granulation
- the stability testing consisted of strength, %recovery, %Met 58 oxidized species, and %related species.
- Table X indicates that strength of rhIL-11, %Met 58 oxidized species and % related of enteric coated tablet did not change at various time points when stored at 2-8°C for 0-6 months.
- enteric coated multiparticulate pellets prepared by fluid bed coating
- accelerated storage conditions 25°C/60% RH for 0-6 months.
- Example 10 Effect of rhIL-11 treatment on chronic diarrhea in HLA-B27 rats
- rhIL-11 multiparticulates contained approximately 1 mg of rhIL-11 per 100 mg multiparticulates, whereas sucrose multiparticulates serves as placebo controls.
- the cumulative effect of single oral doses of enteric-coated rhILL-11 multiparticulates equivalent to 500 ⁇ g/kg rhIL-11 given on alternative days during 2 weeks of treatment was followed by observing the symptoms of diarrhea.
- vehicle-control group consisting of HLA- B27 rats
- the animals were weighed daily during the 2 weeks of oral administration of rhIL-11, and there was no significant change in body weight induced by either rhIL-11 or the placebo.
- All HLA-B27 rats showed clinical symptoms of colitis.
- the stool character was observed daily and characterized as normal, soft, or diarrhea. Scores of 0 for normal, 1 for soft with pellets formed, 2 for soft with no pellet formation, and 3 for diarrhea, were given daily before and during treatment of HLA-B27 rats with rhIL-11 or placebo. Average daily scores were calculated to characterize stool consistency.
- Oral administration of rhIL-11 resulted in significant inhibition of the symptoms of diarrhea, i.e., following the first 9 days of treatment the stool character changed toward normal with soft but normally formed pellets. No changes in stool character were observed in HLA-B27 rats receiving placebo. Likewise, placebo treatment had no effect on the normal stool character in healthy F344 rats.
- Example 11 Effect of rhIL-11 treatment of HLA-B27 rats on intestinal inflammation rhIL-11 was administered orally to test animals as described above in Example 10. Animals were evaluated for intestinal inflammation. All animals were euthanized 4 h after the last administration of rhIL-11 or placebo, and the jejunum and colon were isolated immediately. Myeloperoxidase (MPO), specifically expressed by neutrophils, is considered a marker of inflammatory cell infiltration. The activity of MPO in intestinal tissue extracts was used as an index of inflammation. Full-thickness jejunal and colonic samples (100-150 mg) were taken from the tissue isolated for the contractile experiments and were immediately frozen in liquid nitrogen.
- MPO Myeloperoxidase
- MPO activity was tested in 10- ⁇ l samples using 3,3',5,5'-tetramethylbenzidine Microwell peroxidase substrate system (Sigma Chemical Co., St. Louis, MO) and horseradish peroxidase as a relative standard.
- MPO activity was expressed as equivalent to the activity of the relative standard (nanograms of horseradish peroxidase) converting the same amount of 3,3',5,5'- tetramethylbenzidine substrate for 10 min at room temperature. The data was expressed in nanograms and normalized per gram wet weight of the tissue.
- Example 12 Effect of rhIL-11 treatment of HLA-B27 rats on intestinal inflammation - histological evaluation
- Jejunal and colonic tissue samples were harvested from HLA-B27 rats following the oral administration of rhIL-11 or placebo.
- the specimens were immersed in 10% neutral-buffered formalin, processed, embedded in paraffin, and sectioned at 5- ⁇ m thickness.
- Slide-mounted sections were stained with hematoxylin and eosin and investigated by light microscopy for the presence of ulceration, inflammatory infiltrates, transmural lesions, and fibrosis.
- the slides were examined in a blinded fashion, and each parameter was scored as follows: 0 to 2 for ulceration and fibrosis; 0 to 3 for inflammation and depth of lesions. The absence of pathology was scored as zero.
- a total score was calculated according to the method described by Boughton-Smith et al. (1998) as the sum of the scores of individual parameters (maximum was 10).
- Example 13 Acute effect of rhIL-11 on basal contractile activity
- the baths were filled with Krebs' bicarbonate solution, maintained at 37°C and aerated with 95% O and 5% CO 2 .
- the solution was changed by perfusion at 30-min intervals.
- Each smooth muscle strip was allowed to equilibrate at zero tension for 20 min, followed by consecutive loading with 0.20g force increments until a level of optimal resting tension was achieved. Resting tension was considered to increase with loading. strips were allowed an additional 20 min of equilibration. All experiments were performed at optimal tension and isometric contractions were recorded using a MacLab data acquisition system (AD Instruments Ltd., Castle Hill, Australia).
- Colonic longitudinal muscles isolated from placebo-treated control F344 rats showed low resting tension (2.4 ⁇ 0.3 mN/mm ) with or without occurrence of spontaneous contractions. Resting tension and spontaneous contractions were similar in muscles from F344 and HLA-B27 rats receiving placebo or rhIL-11.
- the addition of rhIL-11 (1-10,000 ng/ml) to the bathing solution showed no acute effects on spontaneous contractility or contractile responses to carbachol (1 ⁇ M) in the colon of Fisher 344 rats or HLA-B27 rats.
- Example 14 Effects of rhIL-11 treatment on receptor-independent intestinal muscle contraction
- Example 15 Effects of rhIL-11 treatment on cholinergic intestinal muscle contraction The effect of rhIL-11 treatment on cholinergic intestinal muscle contraction was examined. Complete dose-response curved to carbachol were obtained in jejunal and colonic longitudinal muscle. Longitudinal muscles isolated from the jejunum of HLA-B27 rats showed abnormal contractile responses. The maximal active tension generated in response to increasing concentrations of carbachol (a nM-10 ⁇ M) was significantly lower in the muscles isolated from placebo-treated HLA-B27 rats compared with placebo-treated Fisher 344 rats. The reduction in contractile responses was accompanied by a shift of the dose-response curve to lower carbachol concentrations.
- the EC 50 for carbachol in jejunal muscles from placebo-treated HLA-B27 rats is significantly lower compared with the EC 50 value obtained in the jejunum of Fisher 344 rats.
- the treatment of HLA-B27 transgenic rats with rhIL-11 resulted in a significant increase in carbachol-induced maximal tension generated by the jejunal muscle. Besides the significant increase, the amplitude of maximal response remained lower than the maximal contraction in muscles from placebo-treated Fisher 344 rats.
- the EC 50 for carbachol in the jejunum of HLA-B27 rats treated with rhIL-11 was significantly reduced compared with placebo-treated HLA-B27 rats and was similar to the EC 50 in the jejunum of Fisher 344 rats.
- the maximal active tension generated in response to carbachol by colonic muscles from placebo-treated HLA-B27 rats was lower than that generated by muscles from placebo-treated Fisher 344 rats.
- the maximal tension induced by carbachol in colonic muscles from rhIL-11 treated HLA-B27 rats was significantly increased compared with placebo-treated HLA-B27 rats and was similar to that in the colon of placebo-treated Fisher 344 rats.
- the concentration-effect curves for carbachol obtained in colonic muscles from F344 and HLA-B27 rats treated with placebo, as well as from HLA-B27 rats treated with rhIL-11 had similar position and did not show significant difference between EC 50 values.
- Example 16 Effects of rhIL-11 treatment on neurally mediated intestinal muscle contraction
- EFS 0.5-ms pulse duration, 5 Hz, 5-s train duration
- EFS induced contractile responses.
- the increase in tension reached maximum during stimulation and decreased to the resting level after the end of the stimulus train.
- Responses to EFS were reproducible throughout the experiment.
- EFS induced nonadrenergic, noncholinergic (NANC) contractile responses of lower amplitude.
- NANC noncholinergic
- Each tablet contains 2.5 mg rhIL-11.
- a 10% overage rhIL-11 is used to compensate for losses during manufacture.
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004536593A JP2006503045A (en) | 2002-09-16 | 2003-09-16 | Delayed release formulations for oral administration of polypeptide therapeutic agents and methods of using the same |
CA002498931A CA2498931A1 (en) | 2002-09-16 | 2003-09-16 | Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same |
EP03756824A EP1545474A1 (en) | 2002-09-16 | 2003-09-16 | Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same |
BR0314356-2A BR0314356A (en) | 2002-09-16 | 2003-09-16 | Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods using the same |
AU2003296413A AU2003296413A1 (en) | 2002-09-16 | 2003-09-16 | Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same |
MXPA05002899A MXPA05002899A (en) | 2002-09-16 | 2003-09-16 | Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US41104002P | 2002-09-16 | 2002-09-16 | |
US60/411,040 | 2002-09-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2004024125A1 true WO2004024125A1 (en) | 2004-03-25 |
Family
ID=31994239
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/029272 WO2004024125A1 (en) | 2002-09-16 | 2003-09-16 | Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same |
Country Status (9)
Country | Link |
---|---|
US (2) | US20040126358A1 (en) |
EP (1) | EP1545474A1 (en) |
JP (1) | JP2006503045A (en) |
CN (1) | CN1688293A (en) |
AU (1) | AU2003296413A1 (en) |
BR (1) | BR0314356A (en) |
CA (1) | CA2498931A1 (en) |
MX (1) | MXPA05002899A (en) |
WO (1) | WO2004024125A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010041096A1 (en) * | 2008-10-10 | 2010-04-15 | Probelte Pharma, S.A. | Orally administrable immunostimulant product for aquaculture |
WO2012065212A1 (en) | 2010-11-19 | 2012-05-24 | Sai Ying Ko | Anti-inflammatory compositions |
US9205130B2 (en) | 2010-02-12 | 2015-12-08 | Centro De Ingenieria Genetica Y Biotecnologia | Orally administrable pharmaceutical pellet of epidermal growth factor |
Families Citing this family (50)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CZ305402B6 (en) * | 2001-02-24 | 2015-09-02 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Xanthine derivatives, their preparation and use in combination therapy |
US7407955B2 (en) | 2002-08-21 | 2008-08-05 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | 8-[3-amino-piperidin-1-yl]-xanthines, the preparation thereof and their use as pharmaceutical compositions |
US7501426B2 (en) | 2004-02-18 | 2009-03-10 | Boehringer Ingelheim International Gmbh | 8-[3-amino-piperidin-1-yl]-xanthines, their preparation and their use as pharmaceutical compositions |
US9895444B2 (en) | 2004-08-25 | 2018-02-20 | Aegis Therapeutics, Llc | Compositions for drug administration |
US8268791B2 (en) * | 2004-08-25 | 2012-09-18 | Aegis Therapeutics, Llc. | Alkylglycoside compositions for drug administration |
US20060046962A1 (en) * | 2004-08-25 | 2006-03-02 | Aegis Therapeutics Llc | Absorption enhancers for drug administration |
EP2457580A1 (en) * | 2004-08-25 | 2012-05-30 | The UAB Research Foundation | Absorption enhancers for drug administration |
US20140162965A1 (en) | 2004-08-25 | 2014-06-12 | Aegis Therapeutics, Inc. | Compositions for oral drug administration |
US20060046969A1 (en) * | 2004-08-25 | 2006-03-02 | Aegis Therapeutics Llc | Antibacterial compositions for drug administration |
US20090047347A1 (en) * | 2005-07-29 | 2009-02-19 | Aegis Therapeutics, Inc. | Compositions for Drug Administration |
DE102004054054A1 (en) | 2004-11-05 | 2006-05-11 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Process for preparing chiral 8- (3-amino-piperidin-1-yl) -xanthines |
DE102005035891A1 (en) | 2005-07-30 | 2007-02-08 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | 8- (3-amino-piperidin-1-yl) -xanthines, their preparation and their use as pharmaceuticals |
PE20080251A1 (en) | 2006-05-04 | 2008-04-25 | Boehringer Ingelheim Int | USES OF DPP IV INHIBITORS |
NZ600394A (en) | 2006-05-04 | 2014-04-30 | Boehringer Ingelheim Int | Polymorphs of a dpp-iv enzyme inhibitor |
EP1852108A1 (en) | 2006-05-04 | 2007-11-07 | Boehringer Ingelheim Pharma GmbH & Co.KG | DPP IV inhibitor formulations |
US8226949B2 (en) | 2006-06-23 | 2012-07-24 | Aegis Therapeutics Llc | Stabilizing alkylglycoside compositions and methods thereof |
US20090069247A1 (en) * | 2006-10-06 | 2009-03-12 | Blake Paterson | Use of tight junction antagonists to treat inflammatory bowel disease |
CA2578647A1 (en) * | 2007-02-15 | 2008-08-15 | University Of Saskatchewan | Reducing post-operative adhesion formation with intraperitoneal glutamine |
WO2009024542A2 (en) * | 2007-08-17 | 2009-02-26 | Boehringer Ingelheim International Gmbh | Purin derivatives for use in the treatment of fab-related diseases |
ES2586032T3 (en) | 2008-03-28 | 2016-10-11 | Hale Biopharma Ventures, Llc | Administration of benzodiazepine compositions |
AR071175A1 (en) * | 2008-04-03 | 2010-06-02 | Boehringer Ingelheim Int | PHARMACEUTICAL COMPOSITION THAT INCLUDES AN INHIBITOR OF DIPEPTIDIL-PEPTIDASA-4 (DPP4) AND A COMPARING PHARMACO |
PE20100156A1 (en) * | 2008-06-03 | 2010-02-23 | Boehringer Ingelheim Int | NAFLD TREATMENT |
US20110201022A1 (en) | 2008-07-30 | 2011-08-18 | Biomarin Pharmaceutical Inc. | Assays for detection of phenylalanine ammonia-lyase and antibodies to phenylalanine ammonia-lyase |
KR20190016601A (en) | 2008-08-06 | 2019-02-18 | 베링거 인겔하임 인터내셔날 게엠베하 | Treatment for diabetes in patients inappropriate for metformin therapy |
UY32030A (en) | 2008-08-06 | 2010-03-26 | Boehringer Ingelheim Int | "TREATMENT FOR DIABETES IN INAPPROPRIATE PATIENTS FOR THERAPY WITH METFORMIN" |
KR101791403B1 (en) * | 2008-08-15 | 2017-10-30 | 베링거 인겔하임 인터내셔날 게엠베하 | Purin derivatives for use in the treatment of FAB-related diseases |
EP2344195A2 (en) | 2008-09-10 | 2011-07-20 | Boehringer Ingelheim International GmbH | Combination therapy for the treatment of diabetes and related conditions |
US20200155558A1 (en) | 2018-11-20 | 2020-05-21 | Boehringer Ingelheim International Gmbh | Treatment for diabetes in patients with insufficient glycemic control despite therapy with an oral antidiabetic drug |
US8440631B2 (en) | 2008-12-22 | 2013-05-14 | Aegis Therapeutics, Llc | Compositions for drug administration |
TWI508965B (en) | 2008-12-23 | 2015-11-21 | Boehringer Ingelheim Int | Salt forms of organic compound |
AR074990A1 (en) | 2009-01-07 | 2011-03-02 | Boehringer Ingelheim Int | TREATMENT OF DIABETES IN PATIENTS WITH AN INAPPROPRIATE GLUCEMIC CONTROL THROUGH METFORMIN THERAPY |
CN102753161A (en) | 2009-11-27 | 2012-10-24 | 贝林格尔.英格海姆国际有限公司 | Treatment of genotyped diabetic patients with dpp-iv inhibitors such as linagliptin |
US8865213B2 (en) * | 2009-12-30 | 2014-10-21 | Usv Limited | Modified release pharmaceutical compositions |
DK3025728T3 (en) * | 2010-02-04 | 2018-10-29 | Biomarin Pharm Inc | PROCEDURE FOR PURIFICATION OF PROKARYOTIC PHENYLALANINE AMMONIAK LYASE VARIATIONS |
US9186392B2 (en) | 2010-05-05 | 2015-11-17 | Boehringer Ingelheim International Gmbh | Combination therapy |
CA2803504C (en) | 2010-06-24 | 2022-08-30 | Boehringer Ingelheim International Gmbh | A combination for diabetes therapy comprising linagliptin and a long-acting insulin |
US9034883B2 (en) | 2010-11-15 | 2015-05-19 | Boehringer Ingelheim International Gmbh | Vasoprotective and cardioprotective antidiabetic therapy |
CN107737100A (en) | 2011-06-14 | 2018-02-27 | 哈尔生物药投资有限责任公司 | The administration of Benzodiazepine composition |
BR112014000938A2 (en) | 2011-07-15 | 2017-01-10 | Boehringer Ingelheim Int | substituted quinazolines, their preparation and their use in pharmaceutical compositions |
US9555001B2 (en) | 2012-03-07 | 2017-01-31 | Boehringer Ingelheim International Gmbh | Pharmaceutical composition and uses thereof |
JP6224084B2 (en) | 2012-05-14 | 2017-11-01 | ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング | Xanthine derivatives as DPP-4 inhibitors for the treatment of glomerular epithelial cell related disorders and / or nephrotic syndrome |
WO2013174767A1 (en) | 2012-05-24 | 2013-11-28 | Boehringer Ingelheim International Gmbh | A xanthine derivative as dpp -4 inhibitor for use in modifying food intake and regulating food preference |
ITMI20132066A1 (en) * | 2013-12-11 | 2015-06-12 | Farmatron Ltd | THERAPEUTIC RELEASE SYSTEMS MODIFIED FOR THE ORAL ADMINISTRATION OF MENTHOL IN THE TREATMENT OF INTESTINAL DISEASES |
EP3110449B1 (en) | 2014-02-28 | 2023-06-28 | Boehringer Ingelheim International GmbH | Medical use of a dpp-4 inhibitor |
CN104434841B (en) * | 2014-12-11 | 2017-06-06 | 顺昊细胞生物技术(天津)股份有限公司 | A kind of site specific DDS for colon multiple cytokine composition |
GB201522186D0 (en) | 2015-12-16 | 2016-01-27 | Singapore Health Services Pte Ltd And Nat University Of Singapore The | Treatment of fibrosis |
BR112018072401A2 (en) | 2016-06-10 | 2019-02-19 | Boehringer Ingelheim International Gmbh | combinations of linagliptin and metformin |
CA3045871A1 (en) | 2016-12-16 | 2018-06-21 | Singapore Health Services Pte Ltd | Il-11 antibodies |
GB201621439D0 (en) | 2016-12-16 | 2017-02-01 | Singapore Health Services Pte Ltd And Nat Univ Of Singapore | IL-11Ra Antibodies |
MX2021008727A (en) | 2019-01-21 | 2021-11-04 | Singapore Health Serv Pte Ltd | Treatment of hepatotoxicity. |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020110593A1 (en) * | 1999-06-04 | 2002-08-15 | Adel Penhasi | Delayed total release two pulse gastrointestinal drug delivery system |
US6531152B1 (en) * | 1998-09-30 | 2003-03-11 | Dexcel Pharma Technologies Ltd. | Immediate release gastrointestinal drug delivery system |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4518584A (en) * | 1983-04-15 | 1985-05-21 | Cetus Corporation | Human recombinant interleukin-2 muteins |
US5215895A (en) * | 1989-11-22 | 1993-06-01 | Genetics Institute, Inc. | Dna encoding a mammalian cytokine, interleukin-11 |
US5292646A (en) * | 1991-02-06 | 1994-03-08 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin and thioredoxin-like molecules |
US5270181A (en) * | 1991-02-06 | 1993-12-14 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin and thioredoxin-like molecules |
DE69425453T2 (en) * | 1993-04-23 | 2001-04-12 | Novartis Ag | Drug delivery device with controlled release |
US5631219A (en) * | 1994-03-08 | 1997-05-20 | Somatogen, Inc. | Method of stimulating hematopoiesis with hemoglobin |
US5496830A (en) * | 1994-09-14 | 1996-03-05 | Johns Hopkins University | Inhibition of hemoflagellates by camptothecin compounds |
US5846958A (en) * | 1995-02-17 | 1998-12-08 | U.S. Bioscience, Inc. | Methods of using aminothiols to promote hematopoietic progenitor cell growth |
US5679339A (en) * | 1995-06-27 | 1997-10-21 | Keith; James | Method of using IL-11 for treating spondyloarthropies |
US6540993B1 (en) * | 1995-06-27 | 2003-04-01 | Wyeth | Method of treating inflammatory bowel disease using a topical formulation of IL-11 |
US6126933A (en) * | 1995-06-27 | 2000-10-03 | Genetics Institute | Methods of treating inflammatory bowel diseases by administering IL-11 |
US6174548B1 (en) * | 1998-08-28 | 2001-01-16 | Andrx Pharmaceuticals, Inc. | Omeprazole formulation |
US6096340A (en) * | 1997-11-14 | 2000-08-01 | Andrx Pharmaceuticals, Inc. | Omeprazole formulation |
UA73092C2 (en) * | 1998-07-17 | 2005-06-15 | Брістол-Майерс Сквібб Компані | Tablets with enteric coating and method for their manufacture |
SE9803772D0 (en) * | 1998-11-05 | 1998-11-05 | Astra Ab | Pharmaceutical formulation |
US6207682B1 (en) * | 1998-12-22 | 2001-03-27 | Novo Nordisk A/S | Modified release formulations containing (R)-1-(10,11-dihydro-5H-Dibenzo[a,d]cyclohepten-5-ylidene)-1propyl)-3-piperidinecarboxylic acid |
-
2003
- 2003-09-16 CA CA002498931A patent/CA2498931A1/en not_active Abandoned
- 2003-09-16 JP JP2004536593A patent/JP2006503045A/en active Pending
- 2003-09-16 WO PCT/US2003/029272 patent/WO2004024125A1/en active Application Filing
- 2003-09-16 EP EP03756824A patent/EP1545474A1/en active Pending
- 2003-09-16 CN CNA038236699A patent/CN1688293A/en active Pending
- 2003-09-16 AU AU2003296413A patent/AU2003296413A1/en not_active Abandoned
- 2003-09-16 MX MXPA05002899A patent/MXPA05002899A/en unknown
- 2003-09-16 BR BR0314356-2A patent/BR0314356A/en not_active IP Right Cessation
- 2003-09-16 US US10/663,264 patent/US20040126358A1/en not_active Abandoned
-
2009
- 2009-04-08 US US12/420,727 patent/US20100062058A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6531152B1 (en) * | 1998-09-30 | 2003-03-11 | Dexcel Pharma Technologies Ltd. | Immediate release gastrointestinal drug delivery system |
US20020110593A1 (en) * | 1999-06-04 | 2002-08-15 | Adel Penhasi | Delayed total release two pulse gastrointestinal drug delivery system |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010041096A1 (en) * | 2008-10-10 | 2010-04-15 | Probelte Pharma, S.A. | Orally administrable immunostimulant product for aquaculture |
US20110225660A1 (en) * | 2008-10-10 | 2011-09-15 | Probelte Pharma, S.A. | Orally administrable immunostimulant product for aquaculture |
US9155708B2 (en) * | 2008-10-10 | 2015-10-13 | Probelte Pharma, S.A. | Orally administrable immunostimulant product for aquaculture |
US9205130B2 (en) | 2010-02-12 | 2015-12-08 | Centro De Ingenieria Genetica Y Biotecnologia | Orally administrable pharmaceutical pellet of epidermal growth factor |
WO2012065212A1 (en) | 2010-11-19 | 2012-05-24 | Sai Ying Ko | Anti-inflammatory compositions |
EP2640411A1 (en) * | 2010-11-19 | 2013-09-25 | Sai Ying Ko | Anti-inflammatory compositions |
EP2640411A4 (en) * | 2010-11-19 | 2013-11-20 | Sai Ying Ko | Anti-inflammatory compositions |
AU2011331901B2 (en) * | 2010-11-19 | 2015-01-22 | BioLingus IP II GmbH | Anti-inflammatory compositions |
US9289493B2 (en) | 2010-11-19 | 2016-03-22 | Biolingus Ip Llc | Method for treating inflammatory conditions with mucosally administered interleukin-2 |
Also Published As
Publication number | Publication date |
---|---|
EP1545474A1 (en) | 2005-06-29 |
BR0314356A (en) | 2005-07-19 |
CN1688293A (en) | 2005-10-26 |
US20040126358A1 (en) | 2004-07-01 |
AU2003296413A1 (en) | 2004-04-30 |
MXPA05002899A (en) | 2005-05-27 |
JP2006503045A (en) | 2006-01-26 |
CA2498931A1 (en) | 2004-03-25 |
US20100062058A1 (en) | 2010-03-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20040126358A1 (en) | Delayed release formulations for oral administration of a polypeptide therapeutic agent and methods of using same | |
EP0667148B1 (en) | Peroral pharmaceutical preparation releasable in lower digestive tract | |
AU713722B2 (en) | Gastrointestinal drug delivery system | |
ES2221188T3 (en) | PHARMACEUTICAL COMPRESSED CONTAINING DIDANOSINE WITH ENTERIC COATING. | |
ES2459018T3 (en) | Close junction effector formulations | |
CA1248023A (en) | Diffusion coated multiple-units dosage form | |
EP0673645B1 (en) | Pharmaceutical composition for targetting drugs to the colon | |
US5891474A (en) | Time-specific controlled release dosage formulations and method of preparing same | |
US6663888B2 (en) | Pulsatile release histamine H2 antagonist dosage form | |
EP1261361B1 (en) | Oral, nasal and pulmonary dosage formulations of copolymer 1 (glatiramer acetate) | |
JP2012514623A (en) | Pharmaceutical composition comprising one or more fumaric acid esters | |
CA2963382A1 (en) | Timed, pulsatile release systems | |
JP2007532689A (en) | Particulate oral galenical formulation for delayed and controlled release of active pharmaceutical ingredients | |
PL181866B1 (en) | Stabilised preparation for oral administration of peptides | |
US20050025824A1 (en) | Pulsatile release histamine H2 antagonist dosage form | |
EP0993305A2 (en) | Composition for enhanced uptake of polar drugs from mucosal surfaces | |
JP2821952B2 (en) | Oral pharmaceutical preparation with lower gastrointestinal release | |
JP5778184B2 (en) | Oral administrable pharmaceutical pellet of epidermal growth factor | |
WO2023166224A1 (en) | Gastroretentive formulations containing protein or peptide |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 167354 Country of ref document: IL |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2498931 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2004536593 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: PA/a/2005/002899 Country of ref document: MX Ref document number: 1029/DELNP/2005 Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2003296413 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 20038236699 Country of ref document: CN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2003756824 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 2003756824 Country of ref document: EP |