CN103142759A - Oral liquid capable of complementing vital essence and tonifying Qi and nourishing blood and tranquilization - Google Patents
Oral liquid capable of complementing vital essence and tonifying Qi and nourishing blood and tranquilization Download PDFInfo
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Abstract
The invention provides oral liquid capable of complementing vital essence and tonifying Qi and nourishing blood and tranquilization. The oral liquid is prepared from the following components in parts by weight: 15 to 80 parts of sea cucumber, 10 to 70 parts of abalone, 10 to 70 parts of sea urchin, 10 to 20 parts of sodium carboxymethylcellulose, 5 to 10 parts of hawthorn, 5 to 10 parts of momordica grosvenori, 10 to 20 parts of barbary wolfberry fruit, 5 to 20 parts of xylooligosaccharide, 5 to 10 parts of chrysanthemum, 0.1 to 0.5 parts of potassium sorbate, and 760 to 965 parts of water. The preparation technology comprises the following steps of: carrying out enzymolysis on sea cucumber, abalone and sea urchin for 4 hours at 38 to 43 DEG C in the presence of 1.0 to 2.0 parts of 10 million U/g proteases; deactivating the enzyme for 30 minutes at 90 to 100 DEG C; extracting hawthorn, momordica grosvenori, barbary wolfberry fruit and chrysanthemum at three times by 6 to 8 times water; filtering; combining the filtrates; concentrating to reach relative density of 1.08; mixing the enzymatic hydrolysate with the traditional Chinese medicine extracting solution; adding sodium carboxymethylcellulose, xylooligosaccharide and potassium sorbate to uniformly mix; adding water until reaching 1000 parts; filtering; and filling. The preparation has the effects of complementing the vital essence and tonifying Qi and nourishing the blood and tranquilization, and is suitable for being applied to the symptoms caused by deficiency of Qi and blood, such as hypoimmunity, insomnia and dreamful sleep, listlessness, dizziness and tinnitus, languidness and hand and foot numbness.
Description
Technical field
The present invention relates to a kind of combination formula, specifically a kind of oral liquid for replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind.
Background technology
Chinese medicine is summarised as essence, gas, god to personal most important material and functional activity, is considered to the basic of life, is the three large key elements of keeping the whole vital movement of human body.Essence is the nutrient substance of human body, dominates the whole growth promoter of human body, the process of reproduction aging; Gas is the power that promotes the internal organs functional activity, and " gas " is the material base that immunity forms, thus gas can not consume, can not be stagnant, how sick stagnant consumption is; God is a series of menses, the thinking activities of human body, and by the heart is led, the heart is the c-in-c of human body, and god occupies its space of top prominence, and the strong manner of the heart is sufficient, and spirited abundance is stiffness of the body, and a little less than manner laxed body, essence was filled, gas foot, god are the assurance of health entirely.Society, along with the acceleration of aged tendency of population and the increase of operating pressure, the quickening of rhythm of life, the increase of bad life style, body is in the state of damage of essence, the deficiency of vital energy, god's consumption often, and then it is not enough to cause energy and blood of human body.The result of insufficiency of vital energy and blood can cause going down of function, causes the pathological changes of senilism.The deficiency of vital energy: namely function decline resistance against diseases is poor.The deficiency of vital energy is aversion to cold and cold limbs, spontaneous perspiration, dizziness and tinnitus, lethargy, tired unable, shortness of breath and palpitation, hypoevolutism.Blood deficiency: lusterless complexion is sallow, xerosis cutis, hair withered, fingernail is dry and cracked, blurring of vision, numb hand and foot, insomnia and dreamful sleep, forgetful cardiopalmus, absentminded.Insufficiency of vital energy and blood also can cause the decline of immunologic function and cause the infection of microorganism, and as flu frequently, wound infection repeatedly, the diseases such as autoimmune disease, tumor have a strong impact on patient's work and life.For improving immunity of organisms, be necessary to develop the preparation of a kind of replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind.
Summary of the invention
Technical problem to be solved by this invention is to provide the oral liquid of a kind of replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind, and not only therapeutic effect is good, and cheap, taking convenience.
the technical solution adopted for the present invention to solve the technical problems is: a kind of for replenishing vital essence and invigorating vital QI, the oral liquid of nourishing blood to tranquillize the mind, it adopts Stichopus japonicus, Hemicentrotus seu Strongylocentrotus, Carnis Haliotidis, sodium carboxymethyl cellulose, potassium sorbate, Fructus Momordicae, Fructus Lycii, oligomeric xylose, Flos Chrysanthemi, Fructus Crataegi, water is raw material, by following quality proportioning: 15～80 parts of Stichopus japonicuss, 10～70 parts of Carnis Haliotidiss, 10～70 parts of Hemicentrotus seu Strongylocentrotuss, 10～20 parts of sodium carboxymethyl cellulose, 5～10 parts of Fructus Crataegis, 5～10 parts of Fructus Momordicaes, 10～20 parts of Fructus Lycii, 5～20 parts of oligomeric xyloses, 5～10 parts of Flos Chrysanthemis, 0.1～0.5 part of potassium sorbate, 760～965 parts, water is made, its preparation technology is: with Stichopus japonicus, Carnis Haliotidis, Hemicentrotus seu Strongylocentrotus with 1.0～2.0 parts of enzymolysis 4h under 38～43 ℃ of conditions of 100,000 U/g protease, then 90～100 ℃ of enzyme denaturing 30min, with Fructus Crataegi, Fructus Momordicae, Fructus Lycii, Flos Chrysanthemi with 6～8 times of water extraction 3 times, filter, merging filtrate, being concentrated into relative density is 1.08, enzymolysis solution is mixed with Chinese medicine extraction liquid, add sodium carboxymethyl cellulose, oligomeric xylose, potassium sorbate dissolving, mix homogeneously, add water to 1000 parts, filtration, fill.
Of the present invention part is mass parts, is the mass units such as this area microgram commonly used, milligram, gram, kilogram.
It is 30～50mL that preparation suggestion day of the present invention takes effective dose, divides and takes for 2 times.
The invention has the beneficial effects as follows: the effect that preparation of the present invention adopts the modern zymolysis technique of traditional Chinese Medicine and Clavicular to play altogether replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind can make that cloudy blood must be mended, the mind must be supported.Wherein Stichopus japonicus benefiting essence-blood, the kidney invigorating gas, the strong essence of gonad of Hemicentrotus seu Strongylocentrotus, the beneficial heart, bone strengthening, enrich blood, Carnis Haliotidis is nourished blood, easing the affected liver, YIN nourishing, heat clearing away, benefit essence, make eye bright, the Fructus Crataegi stomach reinforcing, refresh oneself, QI invigorating and blood, the Fructus Lycii spermatogenesis is mended marrow, enriching yin and nourishing kidney, benefiting vital QI for tranquillizing, the heat clearing away of chrysanthemum powder forimproving wind, and suppressing the hyperactive liver makes eye bright, all medicines share, and are applicable to because of symptoms such as hypoimmunity that insufficiency of vital energy and blood causes, insomnia and dreamful sleep, lethargy, dizziness and tinnitus, tired unable, numb hand and foot.
Compound basis
Stichopus japonicus sweet in the mouth, salty, warm in nature, have benefiting essence-blood, the kidney invigorating gas, the dry effect of intestine moistening, can be used for insufficiency of kidney-YANG and soreness of the waist and knees, aversion to cold and cold limbs, clear urine in large amounts or the blood and essence asthenia that produces, the symptoms such as weak, dryness of the intestine constipation of becoming thin.Contain 30 various active compositions in Stichopus japonicus, human body is played many-sided health care and prevent disease effect.Its composition comprises acid mucopolysaccharide, holothurin, 18 seed amino acids, selenium, zinc, calcium, ferrum, iodine etc.Have health invigorating, adjust the effects such as blood-fat and blood sugar, blood pressure lowering, slow down aging, antitumor.Experimentation shows that acid mucopolysaccharide can obviously increase the weight of mouse spleen, improves phagocytic percentage and the phagocytic index of peritoneal macrophage, the phagocytic function of enhancing body monokaryon one macrophage system.Clinical research shows that acid mucopolysaccharide can significantly reduce the concentration that healthy person in middle and old age organize T-CHOL (TC), serum triglycerides (TG), the concentration of rising ApoA l (AlmA1) and reduction apolipoprotein B (AlmB).Collagen protein in Stichopus japonicus, arginine, mucoitin sulfuric acid, phosphorus, selenium, nicotinic acid etc. have the effects such as life lengthening, allaying tiredness, control skin aging, beauty treatment.At anti-tumor aspect, holothurin can be grown by anticancer, and molybdenum element can suppress esophageal carcinoma, and selenium compound has inhibitory action to pulmonary carcinoma, breast carcinoma, colon cancer.
Hemicentrotus seu Strongylocentrotus; The property flat, salty in the mouth, return liver, kidney, stomach warp.The effect that gonad of Hemicentrotus seu Strongylocentrotus has strong essence, the beneficial heart, bone strengthening, enriches blood.China is among the people regards it as in seafood first-class tonic, have the saying of eating the Hemicentrotus seu Strongylocentrotus nourishing and fit keeping function.Contain protein 41g, fatty 32.7g in every 100g urchin Huang, also contain the nutritional labelings such as vitamin A, each seed amino acid of D and phosphorus, ferrum, calcium.Studies show that, gonad of Hemicentrotus seu Strongylocentrotus can improve immunity of organisms, reduce blood in human body in clearing gallbladder sterin and triglyceride, and the effect of stabilizing blood pressure and prevention of arterial sclerosis is arranged, and therefore, has the function of control cardiovascular and cerebrovascular disease.The zoopery demonstration, the extract of gonad of Hemicentrotus seu Strongylocentrotus can strengthen exercise tolerance, extends the animals survived time.The Hemicentrotus seu Strongylocentrotus extract ripple is that profit rather has the anticancer growth.
Carnis Haliotidis property is flat, and sweet-salty is returned Liver Channel, have nourish blood, easing the affected liver, YIN nourishing, heat clearing away, benefit essence, the function that makes eye bright.The nutritive value of Carnis Haliotidis is very abundant, and the every hectogram Fresh abalone flesh of fish contains protein 23.4g, fatty 3.4g, and inorganic salt calcium 32mg, ferrum 3.0mg also has iodine, zinc, phosphorus and vitamin A. D. E, the B1 etc. of a great deal of.Modern study shows, can extract the bioactive substance of a kind of being known as " Bao Su " in Carnis Haliotidis meat, and it can improve immunity, and the destruction of cancer cells metabolic process can improve tumour inhibiting rate, and does not damage the normal cell of body, and the effect of protection body immune system is arranged.Carnis Haliotidis can the amphicheirality be regulated blood pressure, and reason is that Carnis Haliotidis can " yin nourishing, suppressing the hyperactive liver, reinforce the kidney ", the capable of regulating acth secretion.The content of vitamin E of Carnis Haliotidis is abundant, can prevent cardiovascular and cerebrovascular disease.
The acid of Fructus Crataegi sweet in the mouth, slightly warm in nature has stomach reinforcing, refreshes oneself, the effect of QI invigorating and blood.Fructus Crataegi is rich in the beneficiating ingredients such as the triterpenes olefin(e) acid such as carotene, calcium, oleanolic acid, the acid of bird element, crataegin and flavonoid, energy vasodilator, reinforcement and adjusting cardiac muscle, increase ventricle and heart motional amplitude and crown blood flow volume, have and reduce serum cholesterol and reduce blood pressure and diuresis and calm effect.
The Fructus Momordicae sweet in the mouth, cool in nature; Return lung, spleen channel; Has removing heat from the lung and relieving sorethroat, preventing phlegm from forming and stopping coughing, the effect of loosening bowel to relieve constipation.
The Fructus Lycii sweet in the mouth, property is flat; Return Liver Channel, kidney channel, lung meridian; Fructus Lycii is strengthening the body resistance, and spermatogenesis is mended the good medicine of marrow, enriching yin and nourishing kidney, benefiting vital QI for tranquillizing, building body, slow down aging.Fructus Lycii is improved the effect of immunity of organisms; can QI invigorating essence by force; nourishing the liver and kidney, defying age, only quench one's thirst, warm health, antitumor; simultaneously Fructus Lycii also have reduce blood pressure, the effect of blood fat and blood glucose; the energy atherosclerosis; the liver protecting resists fatty liver, promotes liver cell regeneration.Fructus Lycii can also obviously promote hematopoietic cell proliferation, can make Cervical mucus, strengthens the hemopoietic function of human body, the prevention anemia.Fructus Lycii is rich in the multiple nutritional components such as Fructus Lycii proteoglycan, vitamin C, phosphorus, ferrum, energy tonify deficiency spermatogenesis,
The Flos Chrysanthemi sweet-bitter flavor is slightly cold; Has loose wind heat clearing away, the effect that suppressing the hyperactive liver makes eye bright.Flos Chrysanthemi energy coronary artery dilating, thus myocardial ischemia alleviated,
The function of said preparation:
One. replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind
Stichopus japonicus in this preparation has the effect of benefiting essence-blood, the kidney invigorating gas, the effect that gonad of Hemicentrotus seu Strongylocentrotus has strong essence, the beneficial heart, bone strengthening, enriches blood, Carnis Haliotidis can be nourished blood, easing the affected liver, YIN nourishing, heat clearing away, benefit essence, make eye bright, all medicine phases 5, play altogether the effect of replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind, can make that cloudy blood must be mended, the mind must be supported.
Two. strengthen immunologic function
In preparation of the present invention, contained effective ingredient is by increasing the weight of immune organ, improve thymus index, promote increment and the conversion of splenocyte, strengthen the phagocytic function of mononuclear phagocyte system, interleukin Ⅲ in rising serum (IL-3), interferon (IFN-r) level, improve the generation that antibody-producting cell is counted enhancing antibody, reach the effect that improves body nonspecific immunity and specific immunity.
The specific embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
The invention will be further described below in conjunction with embodiment:
Embodiment 1: a kind of oral liquid for replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind.Adopt Stichopus japonicus 50g, Carnis Haliotidis 45g, Hemicentrotus seu Strongylocentrotus 45g, sodium carboxymethyl cellulose 15g, Fructus Crataegi 8g, Fructus Momordicae 8g, Fructus Lycii 15g, oligomeric xylose 20g, Flos Chrysanthemi 8g, potassium sorbate 0.3g, water 820g to make; Technique: with Stichopus japonicus, Carnis Haliotidis, Hemicentrotus seu Strongylocentrotus with 1.5g38-43 ℃ of enzymolysis 4h of 100,000 U/g protease, then 90-100 ℃ of enzyme denaturing 30min, Fructus Crataegi, Fructus Momordicae, Fructus Lycii, Flos Chrysanthemi with 6-8 times of water extraction 3 times, are filtered merging filtrate, being concentrated into relative density is 1.08, enzymolysis solution is mixed with Chinese medicine extraction liquid, add sodium carboxymethyl cellulose, oligomeric xylose, potassium sorbate dissolving, mix homogeneously, add water to 1000g, oral liquid is made in filtration, fill.
Embodiment 2: a kind of oral liquid for replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind.Adopt Stichopus japonicus 80g, Carnis Haliotidis 30g, Hemicentrotus seu Strongylocentrotus 50g, sodium carboxymethyl cellulose 10g, Fructus Crataegi 5g, Fructus Momordicae 10g, Fructus Lycii 20g, oligomeric xylose 5g, Flos Chrysanthemi 5g, potassium sorbate 0.5g, water 820g to make; Technique: with Stichopus japonicus, Carnis Haliotidis, Hemicentrotus seu Strongylocentrotus with 1.8g38-43 ℃ of enzymolysis 4h of 100,000 U/g protease, then 90-100 ℃ of enzyme denaturing 30min, Fructus Crataegi, Fructus Momordicae, Fructus Lycii, Flos Chrysanthemi with 6-8 times of water extraction 3 times, are filtered merging filtrate, being concentrated into relative density is 1.08, enzymolysis solution is mixed with Chinese medicine extraction liquid, add sodium carboxymethyl cellulose, oligomeric xylose, potassium sorbate dissolving, mix homogeneously, add water to 1000g, oral liquid is made in filtration, fill.
Embodiment 3: a kind of oral liquid for replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind.Adopt Stichopus japonicus 40g, Carnis Haliotidis 70g, Hemicentrotus seu Strongylocentrotus 30g, sodium carboxymethyl cellulose 20g, Fructus Crataegi 10g, Fructus Momordicae 5g, Fructus Lycii 10g, oligomeric xylose 10g, Flos Chrysanthemi 10g, potassium sorbate 0.1g, water 825g to make; Technique: with Stichopus japonicus, Carnis Haliotidis, Hemicentrotus seu Strongylocentrotus with 1.5g38-43 ℃ of enzymolysis 4h of 100,000 U/g protease, then 90-100 ℃ of enzyme denaturing 30min, Fructus Crataegi, Fructus Momordicae, Fructus Lycii, Flos Chrysanthemi with 6-8 times of water extraction 3 times, are filtered merging filtrate, being concentrated into relative density is 1.08, enzymolysis solution is mixed with Chinese medicine extraction liquid, add sodium carboxymethyl cellulose, oligomeric xylose, potassium sorbate dissolving, mix homogeneously, add water to 1000g, oral liquid is made in filtration, fill.
Embodiment 4: zoopery, adopt the oral liquid of making in embodiment 1, and the effect of preparation of the present invention is verified.
1. materials and methods:
1.1 sample
1.2 laboratory animal:
The male balb/c mice of secondary, body weight 18g～22g.
1.3 dosage is selected
Animal is bought back, after normal feedstuff feed 3d, be divided into the 1.7g/kg.bw group by the random packet principle, 3.3g/kg.bw group, 5.0g/kg.bw group (be equivalent to respectively censorship side's recommended dose 10 times, 20 times and 30 times) and matched group, experimental group is with the sample of corresponding dosage, matched group is with the distilled water gavage, gavage dosage is 20mL/kg.bw, once a day, and continuous gavage 30d.
1.4 experimental technique
1.4.1 internal organs/weight ratio pH-value determination pH: after gavage 30d, take the weight of animals continuously, animal is put to death in the cervical vertebra dislocation, takes out spleen, thymus, weighs respectively, calculates internal organs/body weight ratio (representing with mg/10g)
1.4.2 delayed allergy (the sufficient sole of the foot increases rear method), at the 25d of continuous gavage, the SRBC0.2ml immunity of every mouse peritoneal injection 2% is continuously after gavage 4d, measure the thickness of the left back sufficient sole of the foot, survey and average for 3 times, then at measuring point subcutaneous injection 20% (v/v) SRBC, every 20 μ L, after injection, 24h measures the thickness of the left back sufficient sole of the foot, survey and average for 3 times, calculate the difference of sufficient sole of the foot thickness before and after attacking, represent with difference the degree that the mice foot sole of the foot thickens.
1.4.3ConA the mouse spleen lymphocyte transformation experiment (mtt assay) of inducing: continuous gavage 30d, animal is put to death in the cervical vertebra dislocation, and aseptic taking-up spleen tears up, and after crossing 200 eye mesh screens, uses Hank, and s liquid is washed 3 times, is made into 3 * 10 with complete RPMI-1640
6/ mL cell suspension adds every part of splenocyte suspension in 24 hole culture fluid, the 1mL/ hole, and 2 holes/sample, a hole adds 75mLConA liquid (being equivalent to 7.5 μ g/mL), and CO is put in contrast in a hole
2Incubator takes out culture fluid after cultivating 68h, every hole gentle aspiration 0.7mL supernatant, add 0.7mL not contain the RPMI-1640 of calf serum, and 50mLMTT liquid (5mg/mL), to continue to cultivate 4h. and take out culture fluid, every hole adds 1mL acid isopropyl alcohol, piping and druming is dissolved purple crystal fully, lysate is moved in the 2mL cuvette, use 722 spectrophotometers, the place surveys the OD value at the 570nm wavelength.
1.4.4 serum hemolysin is measured (Hemagglutination Method) at the 25d of continuous gavage, the SRBC0.2mL immunity of every mouse peritoneal injection 2%, continuously after gavage 5d, extract eyeball and get blood in centrifuge tube, place 1h, peel off, the centrifugal 10min of 2000r/min collects serum, with normal saline, serum is done doubling dilution, every part is diluted 12 holes, the dilution serum of difference is placed in blood-coagulation-board, every hole 100uL, add again 5% SRBC suspension 100uL, mixing is put observed result after wet 37 ℃ of 3h of box, records the coagulation degree in every hole.The calculating antibody product.
1.4.5 antibody-producting cell detects (Jerne improves slide method) at the 25d of continuous gavage, the SRBC0.2mL immunity of every mouse peritoneal injection 2%, continuously after gavage 5d, animal is put to death in the cervical vertebra dislocation, and aseptic taking-up spleen tears up, after crossing 200 eye mesh screens, use Hank, s liquid is washed 3 times, is made into 5x106/mL cell suspension with complete RPMI-1640 standby.Add the SRBC of 50 μ L10% and the splenocyte suspension that 20uL has prepared in the small test tube that contains 0.5mL (45 ℃-50 ℃) top layer culture medium, mixing, add on the biofilm slide rapidly, after solidifying, the fragmentation button is placed on the slide frame, puts CO
2Incubator is cultivated 1.5h, adds the complement with the dilution of SA buffer in slide frame groove, after continuing to cultivate 1.5h, and counting hemolytic antibody cellulation number.
After 1.4.6 Turnover of Mouse Peritoneal Macrophages is engulfed the continuous gavage 30d of chicken red blood cell experiment (half intracorporal method), every mouse peritoneal injection 20% chicken erythrocyte suspension 2mL, after 30min, the cervical vertebra dislocation is put to death, be fixed on the Mus plate, the medisection abdominal skin, through Intraperitoneal injection normal saline 2mL, rotate Mus plate 1min, sucking-off abdominal cavity washing liquid 1mL, on average splash on 2 microscope slides, put wet 37 ℃ of 30min of box, take out rinsing in normal saline, dry, fixing, the 4%GiemsaPBS 3min that dyes, the distilled water rinsing is dried, microscopy.Calculate phagocytic percentage and phagocytic index.
1.4.7 mice carbon is cleaned up experiment: after gavage 30d, press 0.01mL/g bw and inject the prepared Chinese ink of dilution in 1: 4 from mouse tail vein continuously, timing is immediately got blood 20 μ L respectively at 2min, 10min from mice angular vein clump, is added to contain 2%Na
2CO
3In the test tube of solution, mixing is used spectrophotometer, (uses 0.1%Na in the 600nm mensuration OD of place value
2CO
3Solution is done blank).
1.5 experimental data statistics
Adopt the variance analysis of each experimental group and the comparison of matched group mean that data are processed.
2. result
2.1 the impact of the Mouse Weight of this preparation.
2.1.1 serum hemolysin, antibody-producting cell and delayed allergy mensuration group: Analysis of variance, each dosage group is compared with matched group, initial body weight F=0.2654, P=0.8499, mid-term body weight F=0.1299, P=0.9418, finish body weight F=0.5322, P=0.6252, weight gain value F=0.5899, P=0.6252 all sees Table 1 without significant difference (P＞0.05)
The impact (serum hemolysin, antibody-producting cell and delayed allergy mensuration group) of this preparation of table 1 on Mouse Weight
Turn the mensuration group 2.1.2 engulf chicken red blood cell and pouring: Analysis of variance, each dosage group is compared with matched group, initial body weight F=0.4841, P=0.6953, mid-term body weight F=0.4365, P=0.7280, finish body weight F=0.3904, P=0.7606, weight gain value F=0.1144, P=0.9512 all sees Table 2 without significant difference (P＞0.05)
The impact (engulf chicken red blood cell and pouring turn mensuration group) of this preparation of table 2 on Mouse Weight
2.1.3 carbon is cleaned up the mensuration group: Analysis of variance, each dosage group is compared with matched group, initial body weight F=0.4788, P=0.6991, mid-term body weight F=0.4983, P=0.6858, finish body weight F=0.6582, P=0.5831, weight gain value F=0.2666, P=0.8490 all sees Table 3 without significant difference (P＞0.05)
The impact (carbon clean up mensuration group) of this preparation of table 3 on Mouse Weight
2.2 the impact of this preparation on mice organs/body weight ratio
2.2.1 spleen/body weight ratio: Analysis of variance, F=0.1089, P=0.9544, each dosage group is compared with control group mice, its spleen/body weight ratio there are no significant difference P＞0.05) see Table 4
2.2.2 thymus/body weight ratio: Analysis of variance, F=0.0606, P=0.9802, each dosage group is compared with control group mice, its thymus/body weight ratio there are no significant difference P＞0.05) see Table 4
The impact of this preparation of table 4 on mice organs/body weight ratio
2.3 the impact of this preparation on the mice delayed hypersensitivity.
Analysis of variance,
F=5.5112, P=0.0029. 24h after antigen is attacked, 1.7g/kg.bw group and 3.3g/kg.bw group are compared with control group mice, its swelling degree of the paw is all without significant difference (P＞0.05), 5.0g/kg.bw group is compared with control group mice, there were significant differences for its swelling degree of the paw, and (P＜0.01) sees Table 5
The impact of this preparation of table 5 on the mice delayed hypersensitivity
* represents to compare with matched group (P＜0.01)
2.4 the impact that this preparation produces the mice serum hemolysin.
Analysis of variance, F=5.3477, P=0.0034.1.7g/kg.bw group reaches and compares with control group mice, its antibody product is without significant difference (P＞0.05), and 3.3g/kg.bw group and 5.0g/kg.bw group are compared with control group mice, and there were significant differences for its antibody product, and (P＜0.01) sees Table 6
The impact that this preparation of table 6 produces the mice serum hemolysin
* represents to compare with matched group (P＜0.01)
2.5 antibody-producting cell detects (Jerne improves slide method)
Analysis of variance, F=20.3463, P=0.0000.1.7g/kg.bw group reaches and compares with control group mice, its antibody-producting cell number is without significant difference (P＞0.05), 3.3g/kg.bw group and 5.0g/kg.bw group are compared with control group mice, there were significant differences for its antibody-producting cell number, and (P＜0.01) sees Table 7
The impact of this preparation of table 7 antagonist cellulation number
* represents to compare with matched group (P＜0.01)
2.6 this preparation is engulfed the chicken red blood cell impact to Turnover of Mouse Peritoneal Macrophages
2.6.1 the phagocytic percentage Analysis of variance, F=40.7664, P=0.0000.1.7g/kg.bw group and 3.3g/kg.bw group are compared with control group mice, its peritoneal macrophage is engulfed the chicken red blood cell phagocytic percentage without significant difference (P＞0.05), 5.0g/kg.bw group is compared with control group mice, its peritoneal macrophage is engulfed the chicken red blood cell phagocytic percentage there were significant differences that (P＜0.01) sees Table 8.
2.6.2 the phagocytic index Analysis of variance, F=54.7364, P=0.0000.1.7g/kg.bw group and 3.3g/kg.bw group are compared with control group mice, its peritoneal macrophage is engulfed the chicken red blood cell phagocytic index without significant difference (P＞0.05), 5.0g/kg.bw group is compared with control group mice, its peritoneal macrophage is engulfed the chicken red blood cell phagocytic index there were significant differences that (P＜0.01) sees Table 8.
This preparation of table 8 is engulfed the chicken red blood cell impact to Turnover of Mouse Peritoneal Macrophages
* represents to compare with matched group (P＜0.01)
2.7 mice carbon is cleaned up experiment
Analysis of variance, F=2.9680, P=0.0447.1.7g/kg.bw group and 3.3g/kg.bw group are compared with control group mice, its phagocytic index a is without significant difference (P＞0.05), and the 5.0g/kg.bw group is compared with control group mice, and there were significant differences for its phagocytic index a, and (P＜0.01) sees Table 9.
The impact that this preparation of table 9 is cleaned up mice carbon
3. brief summary
Give respectively mice 1.7g/kg.bw, 3.3g/kg.bw, after this preparation 30d of 5.0g/kg.bw dosage, having no this preparation has harmful effect to body weight and the weightening finish of mice.In mice organs/body weight ratio was measured, each dosage group was compared with control group mice, its internal organs/equal no significant difference of body weight ratio (P＞0.05).In the abnormal experiment of mice delayed, antigen was attacked rear 24 hours, 1.7g/kg.bw group and 3.3g/kg.bw group are compared with control group mice, its swelling degree of the paw is all without significant difference (P＞0.05), 5.0g/kg.bw group is compared with control group mice, there were significant differences for its swelling degree of the paw (P＜0.01).In the experiment of mice serum hemolysin test, 1.7g/kg.bw group reaches and compares with control group mice, its antibody product is without significant difference (P＞0.05), and 3.3g/kg.bw group and 5.0g/kg.bw group are compared with control group mice, and there were significant differences for its antibody product (P＜0.01).Detect in test at antibody-producting cell, 1.7g/kg.bw group reaches and compares with control group mice, its antibody-producting cell number is without significant difference (P＞0.05), 3.3g/kg.bw group and 5.0g/kg.bw group are compared with control group mice, there were significant differences for its antibody-producting cell number (P＜0.01).Turnover of Mouse Peritoneal Macrophages is engulfed in the chicken red blood cell test, 1.7g/kg.bw group and 3.3g/kg.bw group are compared with control group mice, its peritoneal macrophage is engulfed chicken red blood cell phagocytic percentage and phagocytic index without significant difference (P＞0.05), 5.0g/kg.bw group is compared with control group mice, its peritoneal macrophage is engulfed chicken red blood cell phagocytic percentage and phagocytic index, and there were significant differences (P＜0.01).Mice carbon is cleaned up in experiment, 1.7g/kg.bw group and 3.3g/kg.bw group are compared with control group mice, its phagocytic index a is without significant difference (P＞0.05), and the 5.0g/kg.bw group is compared with control group mice, and there were significant differences for its phagocytic index a (P＜0.01).Can think that according to evaluation criterion this inspection product have the function of enhancing immunity.
Embodiment 5: human feeding trial.
Use the oral liquid of preparation in embodiment 3, the raising immunity of organisms effect of preparation of the present invention is tested.
The study subject standard: the crowd of simple hypoimmunity, the age is between 45-65 year, experimental design and grouping: adopt two kinds of control design between self and group.by the routine experimenter of above-mentioned Standard Selection 100, requirement grouping according to randomized, double-blind, be divided into test-meal group and matched group, consider to affect as far as possible the principal element of result, as the age, sex, diet etc., carry out harmonious verification, with the comparability between the assurance group, every group of experimenter's 50 examples, the test-meal group is taken preparation of the present invention 2 times for each person every day, one time 2, matched group is taken placebo, take continuously 60d, the grouping situation sees Table 10, the front two groups of hematurias of test-meal are routine and hepatic and renal function just, breast is saturating, electrocardiogram, B ultrasonic etc. check all within normal range, two groups of subject age sexes, the equal no significant difference of macrophage content (P＞0.05) in lymphocyte in blood and body, has comparability.
As table 10 test-meal is last, data relatively
After test-meal situation see Table 11, table 12.
Table 11 blood testing-lymphocyte
Table 12 blood testing-macrophage
By table 11, as seen from Table 12, take rear 60d, lymphocyte and macrophage content all obviously improve (P＜0.01) before and after the test-meal of test-meal group, and significantly better than matched group, two group differences are (P＜0.01) very significantly.
Result shows, after examination takes preparation 60d of the present invention, self relatively reach before and after the test-meal of test-meal group after test-meal between test-meal group and matched group group relatively, blood medium-sized lymphocyte and macrophage content be obviously improve (P＜0.01) all, so the effect that this invention preparation has enhancing human body immunity power.
Claims (4)
1. the oral liquid of a replenishing vital essence and invigorating vital QI, nourishing blood to tranquillize the mind adopts 760～965 parts of 15～80 parts of Stichopus japonicuss, 10～70 parts of Carnis Haliotidiss, 10～70 parts of Hemicentrotus seu Strongylocentrotuss, 10～20 parts of sodium carboxymethyl cellulose, 5～10 parts of Fructus Crataegis, 5～10 parts of Fructus Momordicaes, 10～20 parts of Fructus Lycii, 5～20 parts of oligomeric xyloses, 5～10 parts of Flos Chrysanthemis, 0.1～0.5 part of potassium sorbate, water to make through enzymolysis, extraction, mixing, filtration, fill.
2. a kind of replenishing vital essence and invigorating vital QI according to claim 1, the oral liquid of nourishing blood to tranquillize the mind, it is characterized in that described preparation technology is: with Stichopus japonicus, Carnis Haliotidis, Hemicentrotus seu Strongylocentrotus is with 1.0～2.0 parts of enzymolysis 4h under 38～43 ℃ of conditions of 100,000 U/g protease, then 90～100 ℃ of enzyme denaturing 30min, with Fructus Crataegi, Fructus Momordicae, Fructus Lycii, Flos Chrysanthemi is with 6～8 times of water extraction 3 times, filter, merging filtrate, being concentrated into relative density is 1.08, enzymolysis solution is mixed with Chinese medicine extraction liquid, add sodium carboxymethyl cellulose, oligomeric xylose, the potassium sorbate dissolving, mix homogeneously, add water to 1000 parts, filter, fill.
3. the oral liquid of a kind of replenishing vital essence and invigorating vital QI according to claim 1, nourishing blood to tranquillize the mind, is characterized in that it is 30～50mL that day takes effective dose, divides and takes for 2 times.
4. the oral liquid of a kind of replenishing vital essence and invigorating vital QI according to claim 1, nourishing blood to tranquillize the mind, is characterized in that enzymolysis enzyme used is protease.
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