DE602004030785D1 - Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren - Google Patents

Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren

Info

Publication number
DE602004030785D1
DE602004030785D1 DE602004030785T DE602004030785T DE602004030785D1 DE 602004030785 D1 DE602004030785 D1 DE 602004030785D1 DE 602004030785 T DE602004030785 T DE 602004030785T DE 602004030785 T DE602004030785 T DE 602004030785T DE 602004030785 D1 DE602004030785 D1 DE 602004030785D1
Authority
DE
Germany
Prior art keywords
kits
nucleic acids
devices
rna
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004030785T
Other languages
English (en)
Inventor
Lawrence J Wangh
Cristina Hartshorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Publication of DE602004030785D1 publication Critical patent/DE602004030785D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
DE602004030785T 2003-05-19 2004-05-18 Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren Active DE602004030785D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47189503P 2003-05-19 2003-05-19
PCT/US2004/015611 WO2004104181A2 (en) 2003-05-19 2004-05-18 Nucleic acid processing methods, kits and devices

Publications (1)

Publication Number Publication Date
DE602004030785D1 true DE602004030785D1 (de) 2011-02-10

Family

ID=33476896

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004030785T Active DE602004030785D1 (de) 2003-05-19 2004-05-18 Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren

Country Status (8)

Country Link
US (2) US7465562B2 (de)
EP (1) EP1631685B1 (de)
JP (1) JP4836795B2 (de)
AT (1) ATE493508T1 (de)
CA (1) CA2526409A1 (de)
DE (1) DE602004030785D1 (de)
ES (1) ES2355899T3 (de)
WO (1) WO2004104181A2 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006073892A2 (en) * 2004-12-30 2006-07-13 Ge Healthcare Bio-Sciences Corp. Phi 15 dna polymerase
JP5073967B2 (ja) * 2006-05-30 2012-11-14 株式会社日立製作所 単一細胞の遺伝子発現定量方法
EP2029777B1 (de) * 2006-05-31 2017-03-08 Sequenom, Inc. Verfahren und zusammensetzungen zur nukleinsäureextraktion aus einer probe
US20080003575A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Methods and composition for RNA extraction
EP2147120B1 (de) * 2007-05-03 2014-04-02 Roche Diagnostics GmbH VERBESSERTE LYSIS UND REVERSE TRANSKRIPTION ZUR mRNA-QUANTIFIZIERUNG
JP4556194B2 (ja) * 2008-02-01 2010-10-06 セイコーエプソン株式会社 生体試料反応方法
JP5367078B2 (ja) 2008-08-01 2013-12-11 エフ.ホフマン−ラ ロシュ アーゲー mRNA定量のための改善された溶解および逆転写
ES2626903T3 (es) * 2008-09-22 2017-07-26 Ventana Medical Systems, Inc. Procesamiento selectivo de material biológico en un sustrato de micromatriz
US20110287951A1 (en) * 2009-01-30 2011-11-24 Emmert-Buck Michael R Methods and systems for purifying, transferring, and/or manipulating nucleic acids
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
JP5766178B2 (ja) 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴThe University Of Chicago SlipChip装置および方法
GB0919159D0 (en) * 2009-11-02 2009-12-16 Sec Dep For Environment Food A Device and apparatus
US9024008B2 (en) * 2010-07-07 2015-05-05 Diagon Ltd. Procedure for the specific isolation of total DNA content of bacterial germs and a kit for this purpose
DE102011078342A1 (de) * 2011-06-29 2013-01-03 Aj Innuscreen Gmbh Rehybridisierendes Sondensystem zur qualitativen und quantitativen Messung von spezifischen Nukleinsäuren in Echtzeit
CN105143449B (zh) 2012-10-26 2018-11-13 株式会社钟化 Rna制备方法
WO2015085194A1 (en) * 2013-12-06 2015-06-11 The Broad Institute, Inc. Enhanced methods of ribonucleic acid hybridization
JP2017209036A (ja) * 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法
DK3568475T3 (da) 2017-01-16 2023-04-17 Spectrum Solutions L L C Nukleinsyrekonserveringsopløsning og fremgangsmåder til anvendelse
CN106867993A (zh) * 2017-02-17 2017-06-20 唐旌生物科技(上海)有限公司 一种逆转录方法、逆转录试剂盒及其应用
CA3120708A1 (en) 2018-11-20 2020-05-28 Spectrum Solutions, Llc Sample collection system including sealing cap and valve
CN112094840A (zh) * 2019-06-17 2020-12-18 南京尧顺禹生物科技有限公司 一种用于基因组片段扩增的微量动物样品基因组dna模板的快速制备方法
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4585623A (en) * 1984-02-27 1986-04-29 Allelix Inc. Device for performing quantitative chemical and immunochemical assays
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JP3494646B2 (ja) * 1992-06-08 2004-02-09 ジェン−プローブ・インコーポレイテッド 単核細胞からの核酸の調製
JPH0646900A (ja) * 1992-07-29 1994-02-22 Tonen Corp 単一容器内での逆転写酵素介在二段階遺伝子増幅反応によるrna遺伝子の増幅検出方法
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US6153425A (en) * 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
DE69836587T2 (de) 1997-04-16 2007-10-11 Applera Corp., Foster City Nukleinsäuresammlung
US6872527B2 (en) * 1997-04-16 2005-03-29 Xtrana, Inc. Nucleic acid archiving
US6111096A (en) 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
CA2310217A1 (en) * 1997-11-26 1999-06-03 Inhibitex, Inc. Extracellular matrix-binding proteins from staphylococcus aureus
CA2312102C (en) * 1997-12-24 2007-09-04 Cepheid Integrated fluid manipulation cartridge
US6087182A (en) * 1998-08-27 2000-07-11 Abbott Laboratories Reagentless analysis of biological samples
US6777210B1 (en) * 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
JP2002529545A (ja) * 1998-11-06 2002-09-10 ユニベルシテ ドゥ モントリオール バイオフィルムを除去するための改良型の殺菌性及び非殺菌性溶液
US6927024B2 (en) * 1998-11-30 2005-08-09 Genentech, Inc. PCR assay
US6458259B1 (en) * 1999-05-11 2002-10-01 Caliper Technologies Corp. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
WO2002020842A1 (en) * 2000-09-06 2002-03-14 Transnetyx, Inc. System, method and apparatus for transgenic and targeted mutagenesis screening
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
AU2004220626B2 (en) * 2003-02-05 2010-07-29 Iquum Inc. Sample processing tubule
US20040259162A1 (en) * 2003-05-02 2004-12-23 Sigma-Aldrich Co. Solid phase cell lysis and capture platform

Also Published As

Publication number Publication date
EP1631685A2 (de) 2006-03-08
EP1631685B1 (de) 2010-12-29
EP1631685A4 (de) 2007-08-29
WO2004104181A3 (en) 2005-12-22
US7465562B2 (en) 2008-12-16
JP4836795B2 (ja) 2011-12-14
ES2355899T3 (es) 2011-04-01
ATE493508T1 (de) 2011-01-15
US20090111170A1 (en) 2009-04-30
US20060275773A1 (en) 2006-12-07
US9279121B2 (en) 2016-03-08
JP2006528002A (ja) 2006-12-14
WO2004104181A2 (en) 2004-12-02
CA2526409A1 (en) 2004-12-02

Similar Documents

Publication Publication Date Title
ATE493508T1 (de) Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
KR102088381B1 (ko) 압타머-기반 다중 검정법
CN101200716B (zh) 使用聚多卡醇和衍生物的核酸分离
Ma et al. Microfluidics for genome-wide studies involving next generation sequencing
ATE314488T1 (de) Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben
US20080176320A1 (en) Selective Lysis of Sperm Cells
CA2354379A1 (en) Method for isolating dna from biological materials
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
ATE549414T1 (de) Verfahren zur isolierung von zellen
KR20030045106A (ko) 비-병원성 또는 병원성 h5 아형 인플루엔자 a 바이러스검출용 키트
BRPI0610092B8 (pt) métodos para a detecção de um analito e de um ácido nucleico em uma amostra, e de sintetização de uma molécula de ácido nucleico ou de análogo de ácido nucleico, kit reagente, uso do mesmo, molécula de ácido nucleico ou de análogo de ácido nucleico e produto associado
WO2001057247A3 (de) Nukleinsäure-isolierung aus stuhlproben und anderen biologischen materialien, die reich an inhibitoren sind
US20180010168A1 (en) One-step procedure for the purification of nucleic acids
CN107406892B (zh) 捕获精子核酸的方法
EP1932913A1 (de) Nukleinsäureisolation mithilfe von Polidocanol und Derivaten davon
ATE487791T1 (de) Verfahren und formulierung zur extraktion von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien
US20110060135A1 (en) Selective Purification of Small RNAs from Mixtures
ATE501279T1 (de) Formulierungen und verfahren zur isolierung von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien und nachfolgende komplexe genanalytik
RU2265058C2 (ru) Способ выявления в образце нуклеиновой кислоты-мишени (варианты)
ATE427991T1 (de) Verfahren zur in vivo quantifizierung von nukleinsauren
CN103695419B (zh) 一种病毒核酸提取试剂
US8993292B2 (en) Methods and systems for differential extraction
ATE419264T1 (de) Verfahren zur kennzeichnung und reinigung von interessierenden nukleinsäuren in einer biologischen probe zur behandlung in einem einzelreaktionsgefäss
Bai et al. Deep sequencing analysis of nucleolar small RNAs: RNA isolation and library preparation
EP3935163A1 (de) Herstellungsverfahren und adaptierte vorrichtung zur filterung von kleinen nukleinsäuren aus biologischen proben