WO1995023843A1 - A process for the reduction of the nucleic acid content of a fungus imperfectus - Google Patents
A process for the reduction of the nucleic acid content of a fungus imperfectus Download PDFInfo
- Publication number
- WO1995023843A1 WO1995023843A1 PCT/GB1995/000368 GB9500368W WO9523843A1 WO 1995023843 A1 WO1995023843 A1 WO 1995023843A1 GB 9500368 W GB9500368 W GB 9500368W WO 9523843 A1 WO9523843 A1 WO 9523843A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- fungus
- temperature
- nucleic acid
- growth medium
- acid content
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/18—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
Definitions
- THIS INVENTION relates to the production of food.
- Suitable fungi are microfungi of the class Fungi Imperfecti.
- a process of this type is described in British Patents Nos. 1,210,356 and 1,346,062 and strains of Fusarium for use in food production are described in British Patent 1,346,061 and in US Patent 4,347.
- British Patent No 1,440,642 discloses a process in which this is achieved by subjecting Fungi Imperfecti to a temperature of 55 to 72°C for a period of at least 60 seconds at a pH of 4.7 to 7.0.
- RNA contents of 1 to 4% from organisms originally containing 7 to 12%. In certain instances RNA contents of the product of less than 1% were disclosed. In the experiments described fungi were generally separated from their growth media and heated in the presence of water or aqueous solutions of NaCl or NH 4 C1 under various conditions.
- Example J the fungi were heated in the presence of their growth medium to a temperature of 64°C, filtered, washed, reslurried in water and spray dried.
- the effect of temperature and duration of heat treatment is shown in Example A of British Patent No 1,440,642.
- 66°C was the final RNA content below the 2% by weight level, which figure was not corrected for biomass loss. The figure based on the final product after biomass loss would have been higher.
- the invention comprises a process in which the nucleic acid content of Fungi Imperfecti is reduced which comprises growing it in the presence of a growth medium, raising its temperature in the presence of the growth medium to above 68°C and preferably at most 80°C for example 69 to 75°C, preferably 70 to 74°C and more preferably above 72°C to 74°C and separating at least part of the growth medium from the fungus.
- Nucleic acid passes from the fungus to the growth medium and the treated fungus has a reduced nucleic acid content.
- the process permits a high retention of fungal components of nutritional value especially protein. It is believed that protein is retained more readily because during nucleic acid removal it is in a substantially coagulated state.
- the nucleic acid content of the treated fungus is preferably at most 3% or more preferably at most 2% by weight, for example about 1% by weight.
- the fungus is suitably an edible strain of Fusarium, for example Fusarium graminearium, Fusarium oxysporum or Fusarium solani.
- IMI International Mycological Institute
- the process may be carried out under a wide range of conditions.
- the pH and constitution of the growth medium should be such that growth of the fungus in it can occur at normal temperatures but if desired it can be modified in the process; for example if heating takes place by steam injection the steam may contain an acidic or alkaline gas, for example S0 2 or NH 3 .
- a pH during the nucleic acid removal of 4 to 8, preferably 4.5 to 7 and more preferably 5 to 6.5 is however desirable. Rapid heating from the growth temperature (which is normally below about 30°C for example 28°C) to the nucleic acid removal temperature is preferred. Suitably such heating should be rapid, preferably taking less than five seconds, more preferably less than one second.
- the product may be washed to remove residual nucleic acids in adhering growth medium but this is not essential if most of the growth medium is removed for example by drainage or filtration.
- EXAMPLE Fusarium Graminearum IMI 145,425 was grown in a continuous fermenter at 28°C in the presence of a growth medium of the following composition at a dilution rate of 0.16 to 0.23/h maintaining a dry cell weight of 14.5g/kg in the fermenter, and with continuous aeration with sterile air containing ammonia gas.
- the pH was controlled at 6 by the continuous addition of ammonia gas.
- the phosphorous was supplied as phosphoric acid, the calcium as the acetate and other salts as the sulphates.
- Dry cell weight calculated as the average of those either side. The dry cell weight was measured every two hours.
Abstract
Description
Claims
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP52274495A JP3593130B2 (en) | 1994-03-01 | 1995-02-22 | Food production |
NZ279872A NZ279872A (en) | 1994-03-01 | 1995-02-22 | Reducing the nucleic acid content of fungus imperfectus by treating it in the presence of its growth medium |
AT95909029T ATE205252T1 (en) | 1994-03-01 | 1995-02-22 | A METHOD FOR REDUCING THE NUCLEIC ACID CONTENT OF FUNGUS IMPERFECTUS |
DE69522554T DE69522554T2 (en) | 1994-03-01 | 1995-02-22 | A METHOD FOR REDUCING THE NUCLEIC ACID CONTENT OF FUNGUS IMPERFECTUS |
EP95909029A EP0749471B1 (en) | 1994-03-01 | 1995-02-22 | A process for the reduction of the nucleic acid content of a fungus imperfectus |
DK95909029T DK0749471T3 (en) | 1994-03-01 | 1995-02-22 | Method of reducing the nucleic acid content of Fungus Imperfectus |
AU17133/95A AU691576B2 (en) | 1994-03-01 | 1995-02-22 | A process for the reduction of the nucleic acid content of a fungus imperfectus |
CA002183648A CA2183648A1 (en) | 1994-03-01 | 1995-02-22 | A process for the reduction of the nucleic acid content of a fungus imperfectus |
US08/696,821 US5739030A (en) | 1994-03-01 | 1995-02-22 | Production of food |
GR20010401315T GR3036540T3 (en) | 1994-03-01 | 2001-09-06 | A process for the reduction of the nucleic acid content of a fungus imperfectus |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9403930.2 | 1994-03-01 | ||
GB9403930A GB9403930D0 (en) | 1994-03-01 | 1994-03-01 | Production of food |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1995023843A1 true WO1995023843A1 (en) | 1995-09-08 |
Family
ID=10751101
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1995/000368 WO1995023843A1 (en) | 1994-03-01 | 1995-02-22 | A process for the reduction of the nucleic acid content of a fungus imperfectus |
Country Status (14)
Country | Link |
---|---|
US (1) | US5739030A (en) |
EP (1) | EP0749471B1 (en) |
JP (1) | JP3593130B2 (en) |
AT (1) | ATE205252T1 (en) |
AU (1) | AU691576B2 (en) |
CA (1) | CA2183648A1 (en) |
DE (1) | DE69522554T2 (en) |
DK (1) | DK0749471T3 (en) |
ES (1) | ES2161864T3 (en) |
GB (1) | GB9403930D0 (en) |
GR (1) | GR3036540T3 (en) |
NZ (1) | NZ279872A (en) |
PT (1) | PT749471E (en) |
WO (1) | WO1995023843A1 (en) |
Cited By (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999030579A1 (en) * | 1997-12-16 | 1999-06-24 | Marlow Foods Limited | Flavouring materials from yeast extracts |
US7635492B2 (en) | 2001-05-04 | 2009-12-22 | Marlow Foods Limited | Edible fungi |
US8993030B2 (en) | 2005-09-23 | 2015-03-31 | Conopco | Low pH aerated products |
GB2518725A (en) * | 2013-07-24 | 2015-04-01 | Marlow Foods Ltd | Edible fungi |
US9005690B2 (en) | 2005-09-23 | 2015-04-14 | Conopco, Inc. | Aerated products with reduced creaming |
US9115349B2 (en) | 2008-10-16 | 2015-08-25 | Conopco, Inc. | Hydrophobin solution containing antifoam |
WO2018002579A1 (en) * | 2016-06-27 | 2018-01-04 | Marlow Foods Limited | Method for reducing the level of rna in biomass comprising filamentous fungi |
WO2018002581A1 (en) | 2016-06-27 | 2018-01-04 | Marlow Foods Limited | Edible fungus |
WO2019046480A1 (en) | 2017-08-30 | 2019-03-07 | Sustainable Bioproducts, Inc. | Edible composition with filamentous fungi and bioreactor system for the cultivation thereof |
WO2019122830A1 (en) | 2017-12-21 | 2019-06-27 | Marlow Foods Limited | Foodstuff |
US10533155B2 (en) | 2016-03-01 | 2020-01-14 | Sustainable Bioproducts, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
GB2579351A (en) | 2018-11-19 | 2020-06-24 | Marlow Foods Ltd | Edible fungus |
WO2020176758A1 (en) | 2019-02-27 | 2020-09-03 | Sustainable Bioproducts, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
US10851396B2 (en) | 2014-07-03 | 2020-12-01 | The Fynder Group, Inc. | Acidophilic fusarium oxysporum strains, methods of their production and methods of their use |
WO2021105704A1 (en) | 2019-11-29 | 2021-06-03 | Marlow Foods Limited | Foodstuffs |
US11118305B2 (en) | 2019-06-18 | 2021-09-14 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
WO2021181084A1 (en) | 2020-03-10 | 2021-09-16 | Marlow Foods Limited | Process to produce ethanol |
WO2021234348A1 (en) | 2020-05-22 | 2021-11-25 | Marlow Foods Limited | Edible fungi |
WO2021234349A1 (en) | 2020-05-22 | 2021-11-25 | Marlow Foods Limited | Edible fungi |
WO2021255435A1 (en) | 2020-06-18 | 2021-12-23 | Marlow Foods Limited | Foodstuff |
WO2024009084A1 (en) | 2022-07-08 | 2024-01-11 | Marlow Foods Limited | Foodstuff |
WO2024009087A1 (en) | 2022-07-08 | 2024-01-11 | Marlow Foods Limited | Foodstuff with first regions and second regions and process for producing that foodstuff |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201501320D0 (en) * | 2015-01-27 | 2015-03-11 | Marlow Foods Ltd | Edible fungi |
Citations (4)
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GB1440642A (en) * | 1973-09-24 | 1976-06-23 | Ranks Hovis Mcdougall Ltd | Production of edible protein containing substances |
US4163692A (en) * | 1977-04-25 | 1979-08-07 | E. I. Du Pont De Nemours And Company | Low phosphate growth of fungal mycelia |
US4501765A (en) * | 1973-02-13 | 1985-02-26 | Ranks Hovis Mcdougall Ltd. | Production of edible protein-containing substances |
WO1991017669A1 (en) * | 1990-05-21 | 1991-11-28 | Imperial Chemical Industries Plc | Production of a proteinaceous composition |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8308162D0 (en) * | 1983-03-24 | 1983-05-05 | Ranks Hovis Mcdougall Plc | Edible protein containing substances |
-
1994
- 1994-03-01 GB GB9403930A patent/GB9403930D0/en active Pending
-
1995
- 1995-02-22 AT AT95909029T patent/ATE205252T1/en not_active IP Right Cessation
- 1995-02-22 DK DK95909029T patent/DK0749471T3/en active
- 1995-02-22 ES ES95909029T patent/ES2161864T3/en not_active Expired - Lifetime
- 1995-02-22 US US08/696,821 patent/US5739030A/en not_active Expired - Lifetime
- 1995-02-22 EP EP95909029A patent/EP0749471B1/en not_active Expired - Lifetime
- 1995-02-22 CA CA002183648A patent/CA2183648A1/en not_active Abandoned
- 1995-02-22 AU AU17133/95A patent/AU691576B2/en not_active Ceased
- 1995-02-22 DE DE69522554T patent/DE69522554T2/en not_active Expired - Lifetime
- 1995-02-22 WO PCT/GB1995/000368 patent/WO1995023843A1/en active IP Right Grant
- 1995-02-22 JP JP52274495A patent/JP3593130B2/en not_active Expired - Lifetime
- 1995-02-22 PT PT95909029T patent/PT749471E/en unknown
- 1995-02-22 NZ NZ279872A patent/NZ279872A/en unknown
-
2001
- 2001-09-06 GR GR20010401315T patent/GR3036540T3/en not_active IP Right Cessation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4501765A (en) * | 1973-02-13 | 1985-02-26 | Ranks Hovis Mcdougall Ltd. | Production of edible protein-containing substances |
GB1440642A (en) * | 1973-09-24 | 1976-06-23 | Ranks Hovis Mcdougall Ltd | Production of edible protein containing substances |
US4163692A (en) * | 1977-04-25 | 1979-08-07 | E. I. Du Pont De Nemours And Company | Low phosphate growth of fungal mycelia |
WO1991017669A1 (en) * | 1990-05-21 | 1991-11-28 | Imperial Chemical Industries Plc | Production of a proteinaceous composition |
Cited By (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999030579A1 (en) * | 1997-12-16 | 1999-06-24 | Marlow Foods Limited | Flavouring materials from yeast extracts |
AU746569B2 (en) * | 1997-12-16 | 2002-05-02 | Marlow Foods Limited | Flavouring materials |
EP1205117A1 (en) * | 1997-12-16 | 2002-05-15 | Marlow Foods Limited | Flavouring materials from yeast extracts |
US6579553B1 (en) | 1997-12-16 | 2003-06-17 | Zeneca Limited | Flavoring materials from filamentous fungi |
US6767571B2 (en) | 1997-12-16 | 2004-07-27 | Zeneca Limited | Flavoring agent from filamentous fungus |
US8672245B2 (en) | 2001-05-04 | 2014-03-18 | Marlow Foods Limited | Edible fungi |
US7635492B2 (en) | 2001-05-04 | 2009-12-22 | Marlow Foods Limited | Edible fungi |
US8993030B2 (en) | 2005-09-23 | 2015-03-31 | Conopco | Low pH aerated products |
US9005690B2 (en) | 2005-09-23 | 2015-04-14 | Conopco, Inc. | Aerated products with reduced creaming |
US9115349B2 (en) | 2008-10-16 | 2015-08-25 | Conopco, Inc. | Hydrophobin solution containing antifoam |
GB2518725B (en) * | 2013-07-24 | 2020-04-15 | Marlow Foods Ltd | Edible fungi |
GB2518725A (en) * | 2013-07-24 | 2015-04-01 | Marlow Foods Ltd | Edible fungi |
US10851396B2 (en) | 2014-07-03 | 2020-12-01 | The Fynder Group, Inc. | Acidophilic fusarium oxysporum strains, methods of their production and methods of their use |
US11505779B2 (en) | 2016-03-01 | 2022-11-22 | The Fynder Group, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
US10787638B2 (en) | 2016-03-01 | 2020-09-29 | The Fynder Group, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
US11015168B2 (en) | 2016-03-01 | 2021-05-25 | The Fynder Group, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
US10533155B2 (en) | 2016-03-01 | 2020-01-14 | Sustainable Bioproducts, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
US10577579B2 (en) | 2016-03-01 | 2020-03-03 | Sustainable Bioproducts, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
US10590379B2 (en) | 2016-03-01 | 2020-03-17 | Sustainable Bioproducts, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
US11001801B2 (en) | 2016-03-01 | 2021-05-11 | The Fynder Group, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
US11261420B2 (en) | 2016-03-01 | 2022-03-01 | The Fynder Group, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
WO2018002581A1 (en) | 2016-06-27 | 2018-01-04 | Marlow Foods Limited | Edible fungus |
GB2557886A (en) * | 2016-06-27 | 2018-07-04 | Marlow Foods Ltd | Edible fungus |
WO2018002579A1 (en) * | 2016-06-27 | 2018-01-04 | Marlow Foods Limited | Method for reducing the level of rna in biomass comprising filamentous fungi |
WO2019046480A1 (en) | 2017-08-30 | 2019-03-07 | Sustainable Bioproducts, Inc. | Edible composition with filamentous fungi and bioreactor system for the cultivation thereof |
US11464251B2 (en) | 2017-08-30 | 2022-10-11 | The Fynder Group, Inc. | Edible foodstuffs and bio reactor design |
US11297866B2 (en) | 2017-08-30 | 2022-04-12 | The Fynder Group, Inc. | Bioreactor system for the cultivation of filamentous fungal biomass |
WO2019122830A1 (en) | 2017-12-21 | 2019-06-27 | Marlow Foods Limited | Foodstuff |
GB2579351A (en) | 2018-11-19 | 2020-06-24 | Marlow Foods Ltd | Edible fungus |
US11419358B2 (en) | 2018-11-19 | 2022-08-23 | Marlow Foods Limited | Edible fungus |
US11039635B2 (en) | 2019-02-27 | 2021-06-22 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles |
WO2020176758A1 (en) | 2019-02-27 | 2020-09-03 | Sustainable Bioproducts, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
EP4292436A3 (en) * | 2019-02-27 | 2024-03-20 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
EP4309505A2 (en) | 2019-02-27 | 2024-01-24 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
EP4292436A2 (en) | 2019-02-27 | 2023-12-20 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
US11478007B2 (en) | 2019-02-27 | 2022-10-25 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
US11272726B2 (en) | 2019-02-27 | 2022-03-15 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
US11432575B2 (en) | 2019-02-27 | 2022-09-06 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
EP3930468A4 (en) * | 2019-02-27 | 2022-08-03 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
US11649586B2 (en) | 2019-06-18 | 2023-05-16 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
US11118305B2 (en) | 2019-06-18 | 2021-09-14 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
US11427957B2 (en) | 2019-06-18 | 2022-08-30 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
US11414815B2 (en) | 2019-06-18 | 2022-08-16 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
US11447913B2 (en) | 2019-06-18 | 2022-09-20 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
US11718954B2 (en) | 2019-06-18 | 2023-08-08 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
WO2021105704A1 (en) | 2019-11-29 | 2021-06-03 | Marlow Foods Limited | Foodstuffs |
WO2021181084A1 (en) | 2020-03-10 | 2021-09-16 | Marlow Foods Limited | Process to produce ethanol |
WO2021234349A1 (en) | 2020-05-22 | 2021-11-25 | Marlow Foods Limited | Edible fungi |
WO2021234348A1 (en) | 2020-05-22 | 2021-11-25 | Marlow Foods Limited | Edible fungi |
GB2597437A (en) | 2020-05-22 | 2022-02-02 | Marlow Foods Ltd | Edible fungi |
GB2597237A (en) | 2020-05-22 | 2022-01-26 | Marlow Foods Ltd | Edible fungi |
WO2021255435A1 (en) | 2020-06-18 | 2021-12-23 | Marlow Foods Limited | Foodstuff |
WO2024009084A1 (en) | 2022-07-08 | 2024-01-11 | Marlow Foods Limited | Foodstuff |
WO2024009087A1 (en) | 2022-07-08 | 2024-01-11 | Marlow Foods Limited | Foodstuff with first regions and second regions and process for producing that foodstuff |
Also Published As
Publication number | Publication date |
---|---|
ES2161864T3 (en) | 2001-12-16 |
GB9403930D0 (en) | 1994-04-20 |
JPH09509580A (en) | 1997-09-30 |
EP0749471B1 (en) | 2001-09-05 |
DE69522554D1 (en) | 2001-10-11 |
US5739030A (en) | 1998-04-14 |
AU1713395A (en) | 1995-09-18 |
GR3036540T3 (en) | 2001-12-31 |
DE69522554T2 (en) | 2002-05-29 |
EP0749471A1 (en) | 1996-12-27 |
ATE205252T1 (en) | 2001-09-15 |
CA2183648A1 (en) | 1995-09-08 |
NZ279872A (en) | 1998-02-26 |
DK0749471T3 (en) | 2001-11-12 |
AU691576B2 (en) | 1998-05-21 |
PT749471E (en) | 2002-02-28 |
JP3593130B2 (en) | 2004-11-24 |
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