WO2004104181A3 - Nucleic acid processing methods, kits and devices - Google Patents

Nucleic acid processing methods, kits and devices Download PDF

Info

Publication number
WO2004104181A3
WO2004104181A3 PCT/US2004/015611 US2004015611W WO2004104181A3 WO 2004104181 A3 WO2004104181 A3 WO 2004104181A3 US 2004015611 W US2004015611 W US 2004015611W WO 2004104181 A3 WO2004104181 A3 WO 2004104181A3
Authority
WO
WIPO (PCT)
Prior art keywords
kits
devices
nucleic acid
processing methods
acid processing
Prior art date
Application number
PCT/US2004/015611
Other languages
French (fr)
Other versions
WO2004104181A2 (en
Inventor
Lawrence J Wangh
Cristina Hartshorn
Original Assignee
Univ Brandeis
Lawrence J Wangh
Cristina Hartshorn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brandeis, Lawrence J Wangh, Cristina Hartshorn filed Critical Univ Brandeis
Priority to JP2006533202A priority Critical patent/JP4836795B2/en
Priority to EP04752605A priority patent/EP1631685B1/en
Priority to US10/557,280 priority patent/US7465562B2/en
Priority to AT04752605T priority patent/ATE493508T1/en
Priority to DE602004030785T priority patent/DE602004030785D1/en
Priority to CA002526409A priority patent/CA2526409A1/en
Publication of WO2004104181A2 publication Critical patent/WO2004104181A2/en
Publication of WO2004104181A3 publication Critical patent/WO2004104181A3/en
Priority to US12/292,037 priority patent/US9279121B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents.
PCT/US2004/015611 2003-05-19 2004-05-18 Nucleic acid processing methods, kits and devices WO2004104181A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2006533202A JP4836795B2 (en) 2003-05-19 2004-05-18 Nucleic acid processing method, kit, and apparatus
EP04752605A EP1631685B1 (en) 2003-05-19 2004-05-18 Nucleic acid processing methods, kits and devices
US10/557,280 US7465562B2 (en) 2003-05-19 2004-05-18 Nucleic acid processing methods, kits and devices
AT04752605T ATE493508T1 (en) 2003-05-19 2004-05-18 METHOD, KITS AND DEVICES FOR PROCESSING NUCLEIC ACIDS
DE602004030785T DE602004030785D1 (en) 2003-05-19 2004-05-18 METHOD, KITS AND DEVICES FOR PROCESSING NUCLEIC ACIDS
CA002526409A CA2526409A1 (en) 2003-05-19 2004-05-18 Nucleic acid processing methods, kits and devices
US12/292,037 US9279121B2 (en) 2003-05-19 2008-11-10 Nucleic acid processing kits and devices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47189503P 2003-05-19 2003-05-19
US60/471,895 2003-05-19

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10557280 A-371-Of-International 2004-05-18
US12/292,037 Division US9279121B2 (en) 2003-05-19 2008-11-10 Nucleic acid processing kits and devices

Publications (2)

Publication Number Publication Date
WO2004104181A2 WO2004104181A2 (en) 2004-12-02
WO2004104181A3 true WO2004104181A3 (en) 2005-12-22

Family

ID=33476896

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/015611 WO2004104181A2 (en) 2003-05-19 2004-05-18 Nucleic acid processing methods, kits and devices

Country Status (8)

Country Link
US (2) US7465562B2 (en)
EP (1) EP1631685B1 (en)
JP (1) JP4836795B2 (en)
AT (1) ATE493508T1 (en)
CA (1) CA2526409A1 (en)
DE (1) DE602004030785D1 (en)
ES (1) ES2355899T3 (en)
WO (1) WO2004104181A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1836297A2 (en) * 2004-12-30 2007-09-26 Amersham Biosciences Corp. Phi 15 dna polymerase
JP5073967B2 (en) * 2006-05-30 2012-11-14 株式会社日立製作所 Single cell gene expression quantification method
WO2007140417A2 (en) * 2006-05-31 2007-12-06 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
US20080003575A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Methods and composition for RNA extraction
ES2365447T3 (en) * 2007-05-03 2011-10-05 Roche Diagnostics Gmbh QUANTIFICATION OF THE INDIVIDUAL CELL mRNA WITH REAL-TIME RT-PCR.
JP4556194B2 (en) * 2008-02-01 2010-10-06 セイコーエプソン株式会社 Biological sample reaction method
WO2010012464A1 (en) 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
WO2010033844A2 (en) * 2008-09-22 2010-03-25 Biomicro, Inc. Selective processing of biological material on a microarray substrate
WO2010088517A1 (en) * 2009-01-30 2010-08-05 The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Methods and systems for purifying transferring and/or manipulating nucleic acids
AU2010229490B2 (en) 2009-03-24 2015-02-12 University Of Chicago Slip chip device and methods
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
GB0919159D0 (en) * 2009-11-02 2009-12-16 Sec Dep For Environment Food A Device and apparatus
BR112012031267A2 (en) * 2010-07-07 2015-10-13 Diagon Kft procedure for the specific isolation of the integral DNA content of bacterial germs and kit for this purpose
DE102011078342A1 (en) * 2011-06-29 2013-01-03 Aj Innuscreen Gmbh Rehybridizing probe system for the qualitative and quantitative measurement of specific nucleic acids in real time
JP6301260B2 (en) 2012-10-26 2018-03-28 株式会社カネカ RNA preparation method
WO2015085194A1 (en) * 2013-12-06 2015-06-11 The Broad Institute, Inc. Enhanced methods of ribonucleic acid hybridization
JP2017209036A (en) * 2016-05-24 2017-11-30 東洋紡株式会社 Improved virus detection method
EP3568475B1 (en) 2017-01-16 2023-03-08 Spectrum Solutions L.L.C. Nucleic acid preservation solution and methods of use
CN106867993A (en) * 2017-02-17 2017-06-20 唐旌生物科技（上海）有限公司 One kind reverses recording method, Reverse Transcriptase kit and its application
WO2020106891A1 (en) 2018-11-20 2020-05-28 Spectrum Solutions, Llc Sample collection system including sealing cap and valve
CN112094840A (en) * 2019-06-17 2020-12-18 南京尧顺禹生物科技有限公司 Rapid preparation method of trace animal sample genome DNA template for genome segment amplification
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639599A (en) * 1992-06-08 1997-06-17 Gen-Probe Incorporated Amplification of nucleic acids from mononuclear cells using iron complexing and other agents

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4585623A (en) * 1984-02-27 1986-04-29 Allelix Inc. Device for performing quantitative chemical and immunochemical assays
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPH0646900A (en) * 1992-07-29 1994-02-22 Tonen Corp Detection of rna gene multiplication by reverse transcriptase-intervening multiplication reaction of gene in one pot process
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US6153425A (en) * 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6872527B2 (en) * 1997-04-16 2005-03-29 Xtrana, Inc. Nucleic acid archiving
JP3666604B2 (en) * 1997-04-16 2005-06-29 アプレラ コーポレーション Nucleic acid archiving
US6111096A (en) * 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
CA2310217A1 (en) * 1997-11-26 1999-06-03 Inhibitex, Inc. Extracellular matrix-binding proteins from staphylococcus aureus
AU758407B2 (en) * 1997-12-24 2003-03-20 Cepheid Integrated fluid manipulation cartridge
US6087182A (en) * 1998-08-27 2000-07-11 Abbott Laboratories Reagentless analysis of biological samples
US6777210B1 (en) * 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
CA2350245A1 (en) * 1998-11-06 2000-05-18 Universite De Montreal Improved bactericidal and non-bactericidal solutions for removing biofilms
US6927024B2 (en) * 1998-11-30 2005-08-09 Genentech, Inc. PCR assay
US6458259B1 (en) * 1999-05-11 2002-10-01 Caliper Technologies Corp. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
AU2001288689A1 (en) * 2000-09-06 2002-03-22 Timothy A. Hodge System, method and apparatus for transgenic and targeted mutagenesis screening
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
EP2574400B1 (en) * 2003-02-05 2016-09-28 Iquum, Inc. Sample processing
CA2524393A1 (en) * 2003-05-02 2004-11-18 Sigma-Aldrich Co. Solid phase cell lysis and capture platform

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639599A (en) * 1992-06-08 1997-06-17 Gen-Probe Incorporated Amplification of nucleic acids from mononuclear cells using iron complexing and other agents

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OBEID P.J. ET AL: "Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction With Cycle Number Selection.", ANAL CHEM., vol. 75, no. 2, 15 January 2003 (2003-01-15), pages 288 - 295, XP001161592 *

Also Published As

Publication number Publication date
DE602004030785D1 (en) 2011-02-10
ES2355899T3 (en) 2011-04-01
WO2004104181A2 (en) 2004-12-02
ATE493508T1 (en) 2011-01-15
JP4836795B2 (en) 2011-12-14
EP1631685A2 (en) 2006-03-08
US20060275773A1 (en) 2006-12-07
EP1631685B1 (en) 2010-12-29
CA2526409A1 (en) 2004-12-02
JP2006528002A (en) 2006-12-14
US9279121B2 (en) 2016-03-08
EP1631685A4 (en) 2007-08-29
US20090111170A1 (en) 2009-04-30
US7465562B2 (en) 2008-12-16

Similar Documents

Publication Publication Date Title
WO2004104181A3 (en) Nucleic acid processing methods, kits and devices
JP4291247B2 (en) Methods for isolating nucleic acids
ES2289611T3 (en) ADSORTION OF NUCLEIC ACIDS IN A SOLID PHASE.
CN101200716B (en) Nucleic acid isolation using polidocanol and derivatives
KR102088381B1 (en) Aptamer-based multiplexed assays
Ma et al. Microfluidics for genome-wide studies involving next generation sequencing
ES2294231T3 (en) IMPROVED METHOD FOR TREATMENT BY BISULPHITE.
CA2354379A1 (en) Method for isolating dna from biological materials
IL145422A0 (en) One step sample preparation and detection of nucleic acids in complex in biological samples
WO2007002567A3 (en) Selective isolation and concentration of nucleic acids from complex samples
US20080176320A1 (en) Selective Lysis of Sperm Cells
WO2001057247A3 (en) Nucleic acid isolation from stool samples and other inhibitor-rich biological materials
EP2094846B1 (en) Use of tde for the isolation of nucleic acids
US20180010168A1 (en) One-step procedure for the purification of nucleic acids
EP2243832A3 (en) Sample preparation method incorporating an alkaline shock
EP1932913B1 (en) Nucleic acid isolation using polidocanol and derivatives
AU2016306451A1 (en) Portable nucleic acid extraction apparatus and method of using the same
CN102834518A (en) Improved recovery of nucleic acids from magnetic glass particles
JP2010523094A (en) Purification method of biomolecule
DK2171098T3 (en) PROCEDURES FOR EXTRACTION AND CLEANING COMPONENTS IN BIOLOGICAL SAMPLES
CN103695419B (en) A kind of Viral nucleic acid extraction reagent
US8993292B2 (en) Methods and systems for differential extraction
WO2020180830A1 (en) Preparation methods and apparatus adapted to filter small nucleic acids from biological samples
WO2006018572A3 (en) Method for labeling and purifying nucleic acids of interest present in a biological sample to be treated in a single reaction vessel
WO2007127564A3 (en) Solid phase based nucleic acid assays combining high affinity capturing and detection by specific hybridization

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2006533202

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2526409

Country of ref document: CA

Ref document number: 2006275773

Country of ref document: US

Ref document number: 10557280

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2004752605

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004752605

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 10557280

Country of ref document: US