WO2005089198A3 - Transgenic soybean seeds having reduced activity of lipoxygenases - Google Patents

Transgenic soybean seeds having reduced activity of lipoxygenases Download PDF

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Publication number
WO2005089198A3
WO2005089198A3 PCT/US2005/008075 US2005008075W WO2005089198A3 WO 2005089198 A3 WO2005089198 A3 WO 2005089198A3 US 2005008075 W US2005008075 W US 2005008075W WO 2005089198 A3 WO2005089198 A3 WO 2005089198A3
Authority
WO
WIPO (PCT)
Prior art keywords
native
seed
nucleic acid
acid fragment
lipoxygenases
Prior art date
Application number
PCT/US2005/008075
Other languages
French (fr)
Other versions
WO2005089198A2 (en
Inventor
S Carl Falco
Brian Mcgonigle
Carl A Maxwell
Original Assignee
Du Pont
S Carl Falco
Brian Mcgonigle
Carl A Maxwell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, S Carl Falco, Brian Mcgonigle, Carl A Maxwell filed Critical Du Pont
Publication of WO2005089198A2 publication Critical patent/WO2005089198A2/en
Publication of WO2005089198A3 publication Critical patent/WO2005089198A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Abstract

The present invention concerns a transgenic soybean plant producing seed having reduced activity of seed lipoxygenases, when compared to a soybean plant expressing wild type activity of native seed lipoxygenases, the transgenic soybean plant having a nucleic acid fragment from at least a portion of at least one soybean seed lipoxygenase gene, wherein the nucleic acid fragment is capable of suppressing expression of native seed lipoxygenases and has been introduced into the soybean plant by transformation. The present invention also concerns a transgenic soybean plant producing seed having reduced activity of seed lipoxygenases and a second native enzyme, when compared to a soybean plant expressing wild type activity of native seed lipoxygenases and the second native enzyme, the transgenic soybean plant having a first nucleic acid fragment from at least a portion of at least one soybean seed lipoxygenase gene, wherein the first nucleic acid fragment is capable of suppressing expression of said native seed lipoxygenases, and a second nucleic acid fragment from at least a portion of at least one second native enzyme gene, wherein the second nucleic acid fragment is capable of suppressing expression of the native second enzyme, wherein the first nucleic acid fragment and the second nucleic acid fragment have been introduced into the soybean plant by transformation, and wherein the second enzyme is selected from the group consisting of an enzyme of the lipid oxidation pathway, fatty acid desaturation pathway, phenylpropanoid pathway, triterpenoid pathway, and combinations thereof. Methods of suppressing wild type activity of native soybean seed lipoxygenases, alone or in combination with suppression of a second native enzyme are also embodied by the present invention.
PCT/US2005/008075 2004-03-12 2005-03-09 Transgenic soybean seeds having reduced activity of lipoxygenases WO2005089198A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US55250204P 2004-03-12 2004-03-12
US60/552,502 2004-03-12
US55624804P 2004-03-25 2004-03-25
US60/556,248 2004-03-25

Publications (2)

Publication Number Publication Date
WO2005089198A2 WO2005089198A2 (en) 2005-09-29
WO2005089198A3 true WO2005089198A3 (en) 2009-04-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/008075 WO2005089198A2 (en) 2004-03-12 2005-03-09 Transgenic soybean seeds having reduced activity of lipoxygenases

Country Status (3)

Country Link
US (1) US20060005276A1 (en)
AR (1) AR048034A1 (en)
WO (1) WO2005089198A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040010818A1 (en) * 2002-05-09 2004-01-15 Mcgonigle Brian Transgenic plants with a suppressed triterpene level
US7781648B2 (en) * 2005-05-18 2010-08-24 Board Of Trustees Of Michigan State University Resistance to soybean aphid in early maturing soybean germplasm
EP3067425A1 (en) 2006-10-31 2016-09-14 E. I. du Pont de Nemours and Company Soybean event dp-305423-1 and constructs for the generation thereof
US8329995B2 (en) * 2007-07-06 2012-12-11 Arcadia Biosciences, Inc. Soybeans with reduced isoflavones
US9133475B2 (en) 2008-11-26 2015-09-15 Board Of Trustees Of Michigan State University Aphid resistant soybean plants
SA109310019B1 (en) 2008-12-30 2014-09-10 Carlsberg Breweries As Barley with Reduced Lipoxygenase Activity
US9598701B2 (en) * 2012-01-23 2017-03-21 E I Du Pont De Nemours And Company Down-regulation of gene expression using artificial MicroRNAs for silencing fatty acid biosynthetic genes
US20140096285A1 (en) * 2012-10-02 2014-04-03 The Board Of Trustees Of The University Of Illinois Soybean-based porcine reproductive and respiratory syndrome virus vaccine and methods for making and using the same
CA2836403A1 (en) 2013-01-04 2014-07-04 Board Of Trustees Of Michigan State University New sources of aphid resistance in soybean plants
CN107099548B (en) * 2017-05-09 2020-11-03 北京大北农生物技术有限公司 Method for improving soybean conversion efficiency
CA3012795C (en) 2018-07-30 2020-05-26 Ruslan MOLCHANOV Bone dust trap
EP3650542A1 (en) * 2018-11-08 2020-05-13 Fraunhofer Gesellschaft zur Förderung der Angewand Method to obtain low triterpene/triterpenoid-containing natural rubber latex
WO2023077199A1 (en) * 2021-11-05 2023-05-11 Commonwealth Scientific And Industrial Research Organisation Modified cereal grain

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6627797B1 (en) * 2000-03-21 2003-09-30 The Texas A&M University System Maize lipoxygenase polynucleotide and methods of use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5013659A (en) * 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
DE69233118T2 (en) * 1991-12-04 2004-04-15 E.I. Du Pont De Nemours And Co., Wilmington FATTY ACID DESATURASE GENES FROM PLANTS
EP1066385A1 (en) * 1998-02-25 2001-01-10 E.I. Du Pont De Nemours & Company Incorporated Plant flavanone-3-hydroxylase
US20040001082A1 (en) * 2002-06-26 2004-01-01 Amir Said System and method of interaction with a computer controlled image display system using a projected light source

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6627797B1 (en) * 2000-03-21 2003-09-30 The Texas A&M University System Maize lipoxygenase polynucleotide and methods of use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK SHIBATA ET AL.: "soybean lipoxygenase-1 mRNA", Database accession no. J02795 *

Also Published As

Publication number Publication date
AR048034A1 (en) 2006-03-22
US20060005276A1 (en) 2006-01-05
WO2005089198A2 (en) 2005-09-29

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